Literature summary for 2.1.1.206 extracted from

Kawamura, T.; Anraku, R.; Hasegawa, T.; Tomikawa, C.; Hori, H.
Transfer RNA methyltransferases from Thermoplasma acidophilum, a thermoacidophilic archaeon (2015), Int. J. Mol. Sci., 16, 91-113 .

Search for more literature for 2.1.1.206

Cloned(Commentary)

Cloned (Comment)	Organism
gene Ta0931, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3) Rosetta 2	Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
additional information	Thermoplasma acidophilum	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	?	-	-
additional information	Thermoplasma acidophilum JCM 9062	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	?	-	-
additional information	Thermoplasma acidophilum AMRC-C165	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	?	-	-
additional information	Thermoplasma acidophilum ATCC 25905	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	?	-	-
additional information	Thermoplasma acidophilum NBRC 15155	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	?	-	-
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	Thermoplasma acidophilum	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	Thermoplasma acidophilum JCM 9062	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	Thermoplasma acidophilum AMRC-C165	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	Thermoplasma acidophilum ATCC 25905	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	Thermoplasma acidophilum NBRC 15155	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	Thermoplasma acidophilum	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	Thermoplasma acidophilum JCM 9062	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	Thermoplasma acidophilum AMRC-C165	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	Thermoplasma acidophilum ATCC 25905	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	Thermoplasma acidophilum NBRC 15155	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	Thermoplasma acidophilum	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	Thermoplasma acidophilum JCM 9062	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	Thermoplasma acidophilum AMRC-C165	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	Thermoplasma acidophilum ATCC 25905	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	Thermoplasma acidophilum NBRC 15155	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	Thermoplasma acidophilum	the methylated nucleotide is identified as pCm	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	Thermoplasma acidophilum JCM 9062	the methylated nucleotide is identified as pCm	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	Thermoplasma acidophilum AMRC-C165	the methylated nucleotide is identified as pCm	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	Thermoplasma acidophilum ATCC 25905	the methylated nucleotide is identified as pCm	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	Thermoplasma acidophilum NBRC 15155	the methylated nucleotide is identified as pCm	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	Q9HJN6	-	-
Thermoplasma acidophilum AMRC-C165	Q9HJN6	-	-
Thermoplasma acidophilum ATCC 25905	Q9HJN6	-	-
Thermoplasma acidophilum JCM 9062	Q9HJN6	-	-
Thermoplasma acidophilum NBRC 15155	Q9HJN6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21 (DE3) Rosetta 2 by anion exchange chromatography, heparin affinity chromatography, and gel filtration, followed by dialysis and ultrafiltration	Thermoplasma acidophilum

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	Thermoplasma acidophilum grows optimally at 59°C and pH 1.9	Thermoplasma acidophilum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	Thermoplasma acidophilum	?	-	-
additional information	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	Thermoplasma acidophilum JCM 9062	?	-	-
additional information	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	Thermoplasma acidophilum AMRC-C165	?	-	-
additional information	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	Thermoplasma acidophilum ATCC 25905	?	-	-
additional information	purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed	Thermoplasma acidophilum NBRC 15155	?	-	-
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	Thermoplasma acidophilum	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	Thermoplasma acidophilum JCM 9062	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	Thermoplasma acidophilum AMRC-C165	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	Thermoplasma acidophilum ATCC 25905	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet	mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position	Thermoplasma acidophilum NBRC 15155	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	Thermoplasma acidophilum	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	Thermoplasma acidophilum JCM 9062	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	Thermoplasma acidophilum AMRC-C165	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	Thermoplasma acidophilum ATCC 25905	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet	initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G	Thermoplasma acidophilum NBRC 15155	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	-	Thermoplasma acidophilum	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	-	Thermoplasma acidophilum JCM 9062	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	-	Thermoplasma acidophilum AMRC-C165	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	-	Thermoplasma acidophilum ATCC 25905	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNA	-	Thermoplasma acidophilum NBRC 15155	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	the methylated nucleotide is identified as pCm	Thermoplasma acidophilum	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	the methylated nucleotide is identified as pCm	Thermoplasma acidophilum JCM 9062	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	the methylated nucleotide is identified as pCm	Thermoplasma acidophilum AMRC-C165	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	the methylated nucleotide is identified as pCm	Thermoplasma acidophilum ATCC 25905	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)	the methylated nucleotide is identified as pCm	Thermoplasma acidophilum NBRC 15155	S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)	-	?

Synonyms

Synonyms	Comment	Organism
Ta0931	-	Thermoplasma acidophilum
transfer RNA methyltransferase	-	Thermoplasma acidophilum
Trm56	-	Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Thermoplasma acidophilum

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Thermoplasma acidophilum

General Information

General Information	Comment	Organism
additional information	Trm56 and an yet unknown tRNA (m7G49) methyltransferase might be included in protein complexes and precipitated by centrifugation at 30000 × g. Thermoplasma acidophilum Trm56 might be part of a large complex with other proteins in living cells	Thermoplasma acidophilum
physiological function	the enzyme Trm56 is responsible for the typical archaeal tRNA modification of 2'-O-methylcytidine at position 56 (Cm56). The Cm modification is only found at position 56 in native tRNALeuUAG. Trm56 methylates both the elongator tRNAMet transcript and its precursor. But the methyl group acceptance activity of the precursor is considerably lower than that of the mature transcript, the methylation by Trm56 occurs mainly after the removal of the intron	Thermoplasma acidophilum