Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D217H | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
D226Y | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
D255Y | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
E185K | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
E202Q | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
E317G | site-directed mutagenesi, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
E63K | site-directed mutagenesis, the mutation causes a twofold increase in activity compared to the wild-type enzyme | Homo sapiens |
G155S | site-directed mutagenesis, the mutation causes an over fourfold decrease in activity compared to the wild-type enzyme | Homo sapiens |
G155V | site-directed mutagenesis, almost inactive mutant | Homo sapiens |
L257V | site-directed mutagenesis, the mutation causes an over fourfold decrease in activity compared to the wild-type enzyme | Homo sapiens |
M72I | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
N264S | site-directed mutagenesi, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
R371H | site-directed mutagenesis, almost inactive mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine38 in tRNAAsp | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Dnmt2 is consistently upregulated in hundreds of tumor samples | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine38 in tRNAAsp | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dnmt2 | - |
Homo sapiens |
TRDMT1 | - |
Homo sapiens |
tRNA-aspartic acid methyltransferase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a highly conserved cytosine-C5 methyltransferase that introduces the C38 methylation of tRNA-Asp in many species, including lower eukaryotes, plants, insects and humans | Homo sapiens |
physiological function | The DNMT2 protein methylates C38 of tRNA-Asp and it has a role in cellular physiology and stress response and its expression levels are altered in cancer tissues | Homo sapiens |