Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Rattus norvegicus | |
cysteine | - |
Rattus norvegicus | |
dithiothreitol | - |
Rattus norvegicus | |
glutathione | required for maximum activity, can be replaced by other reducing agents | Rattus norvegicus |
General Stability | Organism |
---|---|
inactivated during purification, can be reactivated by addition of a thiol | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | oxidized form, S-adenosylmethionine protects against inactivation | Rattus norvegicus | |
N-ethylmaleimide | irreversible | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
S-adenosyl-L-methionine | - |
Rattus norvegicus | |
0.098 | - |
Guanidinoacetate | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
gel filtration | Rattus norvegicus |
26000 | - |
SDS-PAGE, in presence and absence of SDS | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanidinoacetate | Rattus norvegicus | - |
S-adenosyl-L-homocysteine + creatine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
inactivated during purification, can be reactivated by addition of a thiol | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 10 mM phosphate buffer, pH 7.2, 1 mM EDTA, 0.1 M NaCl, concentrated by ultrafiltration | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanidinoacetate | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + creatine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE, gel filtration | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
remains active for 24 h | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rattus norvegicus |