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Literature summary for 2.1.1.187 extracted from

  • Liu, M.; Douthwaite, S.
    Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria (2002), Mol. Microbiol., 44, 195-204.
    View publication on PubMed
Search for more literature for 2.1.1.187

Cloned(Commentary)

Cloned (Comment) Organism
-
 Shewanella putrefaciens
-
 Acinetobacter sp.
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine745 in 23S rRNA Shewanella putrefaciens Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Acinetobacter sp. Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Escherichia coli Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Azotobacter vinelandii Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Klebsiella aerogenes Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Dickeya chrysanthemi Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Proteus mirabilis Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Pseudomonas fluorescens Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Pseudomonas putida Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Pseudomonas stutzeri Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Pseudomonas syringae Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Acinetobacter sp. ADP1 Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. Q9AEP4 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Acinetobacter sp. ADP1 Q9AEP4 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Azotobacter vinelandii C1DSW3 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Dickeya chrysanthemi
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Escherichia coli P36999 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Klebsiella aerogenes
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Proteus mirabilis
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Pseudomonas fluorescens
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Pseudomonas putida
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Pseudomonas stutzeri A4VMZ0 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Pseudomonas syringae Q48F48 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Shewanella putrefaciens
-
 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-

Purification (Commentary)

Purification (Comment) Organism
-
 Shewanella putrefaciens
-
 Acinetobacter sp.
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Shewanella putrefaciens S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Acinetobacter sp. S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Azotobacter vinelandii S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Klebsiella aerogenes S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Dickeya chrysanthemi S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Proteus mirabilis S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Pseudomonas fluorescens S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Pseudomonas putida S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Pseudomonas stutzeri S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro Pseudomonas syringae S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine745 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Acinetobacter sp. ADP1 S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
RlmA ambiguous Escherichia coli
RlmAI
-
 Pseudomonas fluorescens
RlmAI
-
 Klebsiella aerogenes
RlmAI
-
 Pseudomonas putida
RlmAI
-
 Proteus mirabilis
RlmAI
-
 Dickeya chrysanthemi
RlmAI
-
 Shewanella putrefaciens
RlmAI
-
 Azotobacter vinelandii
RlmAI
-
 Pseudomonas stutzeri
RlmAI
-
 Pseudomonas syringae
RlmAI
-
 Acinetobacter sp.
RlmAI
-
 Escherichia coli
rRNA large subunit methyltransferase I
-
 Pseudomonas fluorescens
rRNA large subunit methyltransferase I
-
 Klebsiella aerogenes
rRNA large subunit methyltransferase I
-
 Pseudomonas putida
rRNA large subunit methyltransferase I
-
 Proteus mirabilis
rRNA large subunit methyltransferase I
-
 Dickeya chrysanthemi
rRNA large subunit methyltransferase I
-
 Shewanella putrefaciens
rRNA large subunit methyltransferase I
-
 Azotobacter vinelandii
rRNA large subunit methyltransferase I
-
 Pseudomonas stutzeri
rRNA large subunit methyltransferase I
-
 Pseudomonas syringae
rRNA large subunit methyltransferase I
-
 Acinetobacter sp.
rRNA large subunit methyltransferase I
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas fluorescens
37
-
 assay at Klebsiella aerogenes
37
-
 assay at Pseudomonas putida
37
-
 assay at Proteus mirabilis
37
-
 assay at Dickeya chrysanthemi
37
-
 assay at Shewanella putrefaciens
37
-
 assay at Azotobacter vinelandii
37
-
 assay at Pseudomonas stutzeri
37
-
 assay at Pseudomonas syringae
37
-
 assay at Acinetobacter sp.
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas fluorescens
7.5
-
 assay at Klebsiella aerogenes
7.5
-
 assay at Pseudomonas putida
7.5
-
 assay at Proteus mirabilis
7.5
-
 assay at Dickeya chrysanthemi
7.5
-
 assay at Shewanella putrefaciens
7.5
-
 assay at Azotobacter vinelandii
7.5
-
 assay at Pseudomonas stutzeri
7.5
-
 assay at Pseudomonas syringae
7.5
-
 assay at Acinetobacter sp.
7.5
-
 assay at Escherichia coli