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Literature summary for 2.1.1.186 extracted from

  • Guo, H.Y.; Gao, Z.Q.; Zhang, H.; Wei, Y.; Xu, J.H.; Wang, W.Y.; Yan, A.X.; Dong, Y.H.
    Purification, crystallization and preliminary crystallographic analysis of the 23S rRNA methyltransferase RlmM (Cm2498) from Escherichia coli (2013), Acta Crystallogr. Sect. F, 69, 640-642.
    View publication on PubMed
Search for more literature for 2.1.1.186

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Star cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, using 16% (w/v) polyethylene glycol 3350, 0.05 M citric acid, 0.05 M bis-tris propane pH 5.0 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ADR6
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid resin column chromatography, heparin column chromatography, and Superdex 75 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
Cm2498
-
 Escherichia coli
RlmM
-
 Escherichia coli