BRENDA - Enzyme Database
show all sequences of 2.1.1.173

Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity

Kimura, S.; Ikeuchi, Y.; Kitahara, K.; Sakaguchi, Y.; Suzuki, T.; Suzuki, T.; Nucleic Acids Res. 40, 4071-4085 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene rlmKL, DNA and amino acid sequence determination and analysis, gene rlmL/ycbY has been renamed rlmKL
Escherichia coli
Engineering
Protein Variants
Commentary
Organism
additional information
generation of several base-flipping mutant enzymes lacking m2G2445 formation activity, overview
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
gene rlmL/ycbY has been renamed rlmKL
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
helix 80 and the 12 nt ss region are critical sites necessary for m2G2445 and m7G2069 formation. Transcript 7, which lacks helix 80 and the 12 nt single-strand region, is not methylated at either position
720598
Escherichia coli
?
-
-
-
-
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
-
720598
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
-
720598
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
duplex formation of H74 is not required for the m2G2445 formation. The 29-mer single-stranded transcript 6, which consists of residues C2422 to A2450, can form m2G2445 efficiently
720598
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
RlmKL is a fused methyltransferase consisting of an N-terminal RlmL domain and a C-terminal RlmK domain
Escherichia coli
Synonyms
Synonyms
Commentary
Organism
m2G2445 methyltransferase
-
Escherichia coli
RlmKL
-
Escherichia coli
RlmL
-
Escherichia coli
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene rlmKL, DNA and amino acid sequence determination and analysis, gene rlmL/ycbY has been renamed rlmKL
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Escherichia coli
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
generation of several base-flipping mutant enzymes lacking m2G2445 formation activity, overview
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
helix 80 and the 12 nt ss region are critical sites necessary for m2G2445 and m7G2069 formation. Transcript 7, which lacks helix 80 and the 12 nt single-strand region, is not methylated at either position
720598
Escherichia coli
?
-
-
-
-
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
-
720598
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
-
720598
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
duplex formation of H74 is not required for the m2G2445 formation. The 29-mer single-stranded transcript 6, which consists of residues C2422 to A2450, can form m2G2445 efficiently
720598
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
RlmKL is a fused methyltransferase consisting of an N-terminal RlmL domain and a C-terminal RlmK domain
Escherichia coli
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
deletion of rlmL/ycbY results in a slight growth reduction phenotype
Escherichia coli
additional information
RlmKL is involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD
Escherichia coli
physiological function
cooperative methylation of helix 74 by RlmKL plays a key role in the efficient assembly of the 50S subunit, RlmKL enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification. RlmKL has an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. Methyltransferase RlmL, encoded by rlmL/ycbY, catalyzes S-adenosyl-L-methionine-dependent m2G2445 formation
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
deletion of rlmL/ycbY results in a slight growth reduction phenotype
Escherichia coli
additional information
RlmKL is involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD
Escherichia coli
physiological function
cooperative methylation of helix 74 by RlmKL plays a key role in the efficient assembly of the 50S subunit, RlmKL enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification. RlmKL has an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. Methyltransferase RlmL, encoded by rlmL/ycbY, catalyzes S-adenosyl-L-methionine-dependent m2G2445 formation
Escherichia coli
Other publictions for EC 2.1.1.173
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720987
Kita
Crystal structure of a putativ ...
Escherichia coli
Protein Pept. Lett.
20
530-537
2013
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720598
Kimura
Base methylations in the doubl ...
Escherichia coli
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40
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1
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2
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4
1
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1
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3
3
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720601
Wang
Structure of the bifunctional ...
Escherichia coli, Streptococcus mutans
Nucleic Acids Res.
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2
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3
3
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718495
Wang
Purification, crystallization ...
Escherichia coli
Acta Crystallogr. Sect. F
66
1484-1486
2010
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689247
Sergiev
Ribosomal RNA guanine-(N2)-met ...
Escherichia coli
Nucleic Acids Res.
35
2295-2301
2007
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675411
Lesnyak
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Escherichia coli
J. Mol. Biol.
364
20-25
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2
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704261
Reddy
Characterization of the uup lo ...
Escherichia coli
J. Bacteriol.
182
1978-1986
2000
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