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Literature summary for 2.1.1.160 extracted from

  • Li, M.; Sun, Y.; Pan, S.; Deng, W.; Yu, O.; Zhang, Z.
    Engineering a novel biosynthetic pathway in Escherichia coli for the production of caffeine (2017), RSC Adv., 7, 56382-56389 .
No PubMed abstract available

Application

Application Comment Organism
synthesis production of caffeine in metabolically engineered Escherichia coli by the expression of caffeine synthase TCS1. Caffeine accumulation is increased using higher-level expression of the target enzymes, and enhancement of xanthine and S-adenosyl-L-methionine biosynthesis. The final strain produces up to 21.46 mg/l caffeine from 20 g/l of glucose in shake flask culture, yielding caffeine up to 2.96 mg/g glucose Camellia sinensis

Organism

Organism UniProt Comment Textmining
Camellia sinensis Q9FZN8
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Synonyms

Synonyms Comment Organism
TCS1
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Camellia sinensis