Literature summary for 2.1.1.143 extracted from

Neelakandan, A.K.; Nguyen, H.T.; Kumar, R.; Tran, L.S.; Guttikonda, S.K.; Quach, T.N.; Aldrich, D.L.; Nes, W.D.; Nguyen, H.T.
Molecular characterization and functional analysis of Glycine max sterol methyl transferase 2 genes involved in plant membrane sterol biosynthesis (2010), Plant Mol. Biol., 74, 503-518.

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Cloned(Commentary)

Cloned (Comment)	Organism
genes SMT2-1 and SMT2-2, generation of a beta-conglycinin-AtHMGR1:glycinin-GmSMT1 construct and a glycinin-GmSMT1-pZY101-Asc construct, and of a a three gene casette vector beta-conglycinin-AtHMGR1:glycinin-GmSMT1:beta-phaseolin-GmSMT2-2, overview. cis-Acting elements occur in SMT2 upstream sequences, motifs responsible for basal and regulated expression, overview. Expression of SMT2 in Arabidopsis thaliana ecotype Columbia plants, expression of genes SMT2-1 and SMT2-2 complements the SMT deficient cvp1 mutant Arabidopsis plants. The transgenic plants harboring the SMT2-1 promoter show enzyme-GUS expression predominantly in the cotyledons and the base of the hypocotyls in seedlings, and the tips of young leaves. Transgenic plants with the SMT2-2 promoter had a similar expression pattern, but with strong enzyme-Gus expression throughout the leaves and stem, specifically in the vascular tissue, in both etiolated and light-grown plants	Glycine max

Cloned (Comment)

Organism

genes SMT2-1 and SMT2-2, generation of a beta-conglycinin-AtHMGR1:glycinin-GmSMT1 construct and a glycinin-GmSMT1-pZY101-Asc construct, and of a a three gene casette vector beta-conglycinin-AtHMGR1:glycinin-GmSMT1:beta-phaseolin-GmSMT2-2, overview. cis-Acting elements occur in SMT2 upstream sequences, motifs responsible for basal and regulated expression, overview. Expression of SMT2 in Arabidopsis thaliana ecotype Columbia plants, expression of genes SMT2-1 and SMT2-2 complements the SMT deficient cvp1 mutant Arabidopsis plants. The transgenic plants harboring the SMT2-1 promoter show enzyme-GUS expression predominantly in the cotyledons and the base of the hypocotyls in seedlings, and the tips of young leaves. Transgenic plants with the SMT2-2 promoter had a similar expression pattern, but with strong enzyme-Gus expression throughout the leaves and stem, specifically in the vascular tissue, in both etiolated and light-grown plants

Glycine max

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of transgenic Arabidopsis thaliana plants expressing gene SMT-2 from Glycine max under control of the CaMV S35 promoter, functional complementation of SMT deficient cvp1 mutant Arabidopsis plants. Role of SMT2 in Arabidopsis seed sterol accumulation, changes in the total sterol content in transgenic seeds, phenotypes, overview	Glycine max

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Glycine max	D2D5G3	SMT2-1	-
Glycine max	D2D5G4	SMT2-2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
seedling	-	Glycine max	-

Synonyms

Synonyms	Comment	Organism
SMT2	-	Glycine max
sterol methyl transferase 2	-	Glycine max

Expression

Organism	Comment	Expression
Glycine max	environmental cues, including dehydration, cold, and abscisic acid induce differential changes in transcript levels of the SMT2 during soybean seedling growth	additional information

General Information

General Information	Comment	Organism
metabolism	SMT2 is a rate-limiting enzyme in channeling the carbon flux downstream towards C-24 alkyl sterol synthesis	Glycine max
physiological function	SMT-1 and SMT-2 play a role in regulation of 24-alkyl sterol-controlled plant physiology. The reaction catalyzed by the SMT2 enzyme diverts carbon flux towards the C-24 ethyl sterols, which represents the final segment of phytosterol biosynthesis. SMT-2 plays a role in seed sterol accumulation	Glycine max