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Literature summary for 2.1.1.141 extracted from

  • Preuss, A.; Augustin, C.; Figueroa, C.R.; Hoffmann, T.; Valpuesta, V.; Sevilla, J.F.; Schwab, W.
    Expression of a functional jasmonic acid carboxyl methyltransferase is negatively correlated with strawberry fruit development (2014), J. Plant Physiol., 171, 1315-1324.
    View publication on PubMed
Search for more literature for 2.1.1.141

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells and Saccharomyces cerevisiae strain INV Sc1 Fragaria x ananassa
expressed in Escherichia coli BL21 (DE3) pLysS cells and Saccharomyces cerevisiae strain INV Sc1 Fragaria vesca

Protein Variants

Protein Variants Comment Organism
L245DEL the deletion mutant is inactive Fragaria x ananassa
L245DEL the deletion mutant is inactive Fragaria vesca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0223
-
 jasmonate at pH 5.5 and 30°C Fragaria x ananassa
0.0223
-
 jasmonate at pH 5.5 and 30°C Fragaria vesca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + jasmonate Fragaria x ananassa
-
 S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?
S-adenosyl-L-methionine + jasmonate Fragaria vesca
-
 S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?

Organism

Organism UniProt Comment Textmining
Fragaria vesca
-
-
-
Fragaria x ananassa
-
 cultivar Elsanta
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography Fragaria x ananassa
nickel affinity chromatography Fragaria vesca

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Fragaria x ananassa
-
fruit
-
 Fragaria vesca
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
 with benzoate as substrate, at pH 5.5 and 30°C Fragaria x ananassa
0.11
-
 with benzoate as substrate, at pH 5.5 and 30°C Fragaria vesca
0.18
-
 with salicylate as substrate, at pH 5.5 and 30°C Fragaria x ananassa
0.18
-
 with salicylate as substrate, at pH 5.5 and 30°C Fragaria vesca
7
-
 with jasmonate as substrate, at pH 5.5 and 30°C Fragaria x ananassa
7
-
 with jasmonate as substrate, at pH 5.5 and 30°C Fragaria vesca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme activity with benzoate and salicylate as substrates is less than 1.5% of that with jasmonate Fragaria x ananassa ?
-
 ?
additional information the enzyme activity with benzoate and salicylate as substrates is less than 1.5% of that with jasmonate Fragaria vesca ?
-
 ?
S-adenosyl-L-methionine + jasmonate
-
 Fragaria x ananassa S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?
S-adenosyl-L-methionine + jasmonate
-
 Fragaria vesca S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?

Synonyms

Synonyms Comment Organism
jasmonic acid carboxyl methyltransferase
-
 Fragaria x ananassa
jasmonic acid carboxyl methyltransferase
-
 Fragaria vesca
JMT
-
 Fragaria x ananassa
JMT
-
 Fragaria vesca
S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase
-
 Fragaria x ananassa
S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase
-
 Fragaria vesca

Expression

Organism Comment Expression
Fragaria x ananassa the enzyme's transcript levels decrease steadily during strawberry fruit ripening down
Fragaria vesca the enzyme's transcript levels decrease steadily during strawberry fruit ripening down