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Literature summary for 2.1.1.11 extracted from

  • Sawicki, A.; Willows, R.D.
    S-adenosyl-L-methionine:magnesium-protoporphyrin IX O-methyltransferase from Rhodobacter capsulatus: mechanistic insights and stimulation with phospholipids (2007), Biochem. J., 406, 469-478.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Phospholipids increase enzymatic activity 3-4fold Rhodobacter capsulatus

Cloned(Commentary)

Cloned (Comment) Organism
derived from plasmid pRPS404, consisting of a 46 kb pair section of the photosynthetic gene cluster, overexpression of His6-tagged protein in Escherichia coli, strain BL21DE3, expression plasmid pHisBchM Rhodobacter capsulatus

General Stability

General Stability Organism
phosphatidylglycerol stabilizes enzymatic activity causing disaggregation to lower molecular mass forms Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
cobalt II-protoporphyrin non-substrate metalloporphyrin, competitive, assay described Rhodobacter capsulatus
magnesium protoporphyrin monomethyl ester non-competitive product inhibition Rhodobacter capsulatus
manganese III-protoporphyrin non-substrate metalloporphyrin, non-competitive or un-competitive, assay described Rhodobacter capsulatus
additional information higher magnesium ion concentrations above 3.8 mM inhibit enzyme activity in presence or absence of magnesium-chelatase subunits or BSA Rhodobacter capsulatus
S-adenosyl-L-homocysteine non-competitive product inhibition Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
magnesium deuteroporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
0.0008
-
zinc protoporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
0.001
-
magnesium mesoporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
0.045
-
S-adenosyl-L-methionine previously determined constant Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
His6-tagged protein, gel filtration, SDS-PAGE Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + magnesium mesoporphyrin Rhodobacter capsulatus
-
S-adenosyl-L-homocysteine + magnesium mesoporphyrin monomethyl ester
-
?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus P26236
-
-

Purification (Commentary)

Purification (Comment) Organism
from inclusion bodies, gel filtration and SDS-PAGE Rhodobacter capsulatus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Rhodobacter capsulatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
effect of detergents and alcohols on activity analyzed, no stimulatory effect by magnesium-chelatase subunits, heat-stable stimulatory component present in S-adenosyl-L-methionine synthetase found to be a phospholipid, kinetic analysis shows a random sequential reaction mechanism Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + magnesium deuteroporphyrin in situ generated S-adenosyl-L-methionine substrate reveals higher enzymatic activity in assay, substrate specificity limited to 5-co-ordinate squarepyramidal metalloporphyrins Rhodobacter capsulatus S-adenosyl-L-homocysteine + magnesium deuteroporphyrin monomethyl ester
-
?
S-adenosyl-L-methionine + magnesium mesoporphyrin
-
Rhodobacter capsulatus S-adenosyl-L-homocysteine + magnesium mesoporphyrin monomethyl ester
-
?
S-adenosyl-L-methionine + magnesium mesoporphyrin in situ generated S-adenosyl-L-methionine substrate reveals higher enzymatic activity in assay Rhodobacter capsulatus S-adenosyl-L-homocysteine + magnesium mesoporphyrin monomethyl ester
-
?
S-adenosyl-L-methionine + magnesium protoporphyrin in situ generated S-adenosyl-L-methionine substrate reveals higher enzymatic activity in assay Rhodobacter capsulatus S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester
-
?
S-adenosyl-L-methionine + zinc protoporphyrin in situ generated S-adenosyl-L-methionine substrate reveals higher enzymatic activity in assay, substrate specificity limited to 5-co-ordinate squarepyramidal metalloporphyrins Rhodobacter capsulatus S-adenosyl-L-homocysteine + zinc protoporphyrin monomethyl ester
-
?

Subunits

Subunits Comment Organism
More disaggregation to lower molecular mass forms, monomeric to multimeric species, gel filtration Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
BchM
-
Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodobacter capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
magnesium deuteroporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
0.018
-
zinc protoporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
0.04
-
magnesium protoporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
slight preference for Tris/HCl buffer over Tricine/NaOH Rhodobacter capsulatus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 within activity range Rhodobacter capsulatus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00045
-
magnesium protoporphyrin monomethyl ester non-competitive product inhibition, magnesium deuteroporphyrin as substrate Rhodobacter capsulatus
0.00061
-
magnesium protoporphyrin monomethyl ester non-competitive product inhibition, S-adenosyl-L-methionine as substrate Rhodobacter capsulatus
0.121
-
S-adenosyl-L-homocysteine non-competitive, magnesium protoporphyrin as substrate Rhodobacter capsulatus
0.141
-
S-adenosyl-L-homocysteine non-competitive, S-adenosyl-L-methionine as substrate Rhodobacter capsulatus