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Literature summary for 1.9.6.1 extracted from

  • Eltarahony, M.; Zaki, S.; Kheiralla, Z.; Abd-El-Haleem, D.
    NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis (2018), Biotechnol. Rep., 18, e00257 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.9.6.1

Application

Application Comment Organism
analysis immobilization of whole cells and NAP enzyme for simultaneous bioremediation and bionanoparticles synthesis. The immobilized cells completely remove NO3- upon 192 h with Ag nanoparticles synthesis ranging from 23.26 to 58.14 nm. Immobilized NAP exhibits lower efficiency with 28.6% of NO3- elimination within 288 h and large aggregated Ag nanoparticles ranging from 94.44 nm to 172.22 nm Achromobacter sp. MMT

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 20.2% residual activity Achromobacter sp. MMT
Ag+ 1 mM, 10.2% residual activity Achromobacter sp. MMT
azide 1 mM, 50.7% residual activity Achromobacter sp. MMT
Cd2+ 1 mM, 10.0% residual activity Achromobacter sp. MMT
Co2+ 1 mM, 66.3% residual activity Achromobacter sp. MMT
Cr3+ 1 mM, 45.5% residual activity Achromobacter sp. MMT
cyanide 1 mM, 39.7% residual activity Achromobacter sp. MMT
dithiothreitol 1 mM, 43.7% residual activity Achromobacter sp. MMT
H2O2 1 mM, 37.3% residual activity Achromobacter sp. MMT
Hg2+ 1 mM, 12.4% residual activity Achromobacter sp. MMT
Mn2+ 1 mM, 72.7% residual activity Achromobacter sp. MMT
additional information not inhibitory: urea, phenol Achromobacter sp. MMT
Ni2+ 1 mM, 64.7% residual activity Achromobacter sp. MMT
Pb2+ 1 mM, 53.1% residual activity Achromobacter sp. MMT
PMSF 1 mM, 36.8% residual activity Achromobacter sp. MMT
thiocyanate 1 mM, 62% residual activity Achromobacter sp. MMT

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14.02
-
 nitrate pH 6-7, 30-40°C Achromobacter sp. MMT

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Achromobacter sp. MMT
-
-

Organism

Organism UniProt Comment Textmining
Achromobacter sp. MMT
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
414
-
 40°C, pH 6-7 Achromobacter sp. MMT
487
-
 30°C, pH 6-7 Achromobacter sp. MMT

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + reduced benzyl viologen
-
 Achromobacter sp. MMT nitrite + oxidized benzyl viologen + H2O
-
 ?

Synonyms

Synonyms Comment Organism
NAP
-
 Achromobacter sp. MMT

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
 Achromobacter sp. MMT

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Achromobacter sp. MMT