Protein Variants | Comment | Organism |
---|---|---|
K56H | 11% of wild-type activity at pH 7 | Cereibacter sphaeroides f. sp. denitrificans |
K56M | 5% of wild-type activity at pH 7 | Cereibacter sphaeroides f. sp. denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
nitrate | mutant K56M, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans | |
0.05 | - |
nitrate | wild-type, 25°C, pH 5 | Cereibacter sphaeroides f. sp. denitrificans | |
0.075 | - |
nitrate | mutant K56M, 25°C, pH 7 | Cereibacter sphaeroides f. sp. denitrificans | |
0.11 | - |
nitrate | mutant K56H, 25°C, pH 5 | Cereibacter sphaeroides f. sp. denitrificans | |
0.14 | - |
nitrate | mutant K56H, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans | |
0.17 | - |
nitrate | wild-type, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans | |
0.25 | - |
nitrate | wild-type, 25°C, pH 7 | Cereibacter sphaeroides f. sp. denitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Cereibacter sphaeroides f. sp. denitrificans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | relative metal content is around 1 Mo atom to 6 Fe atoms | Cereibacter sphaeroides f. sp. denitrificans | |
Molybdenum | the active site is a Mo bis-(pyranopterin guanine dinucleotide), relative metal content is around 1 Mo atom to 6 Fe atoms | Cereibacter sphaeroides f. sp. denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides f. sp. denitrificans | O88111 and O88160 | O88111 i.e. catalytic subunit NapA, O88160 i.e. electron transfer subunit NapB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + reduced benzyl viologen | - |
Cereibacter sphaeroides f. sp. denitrificans | nitrite + oxidized benzyl viologen + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NapAB | - |
Cereibacter sphaeroides f. sp. denitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
nitrate | mutant K56M, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans | |
22 | - |
nitrate | mutant K56H, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans | |
61 | - |
nitrate | wild-type, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | a [4Fe-4S] cluster and two c-type hemes form an intramolecular electron transfer chain that deliver electrons to the active site. Residue lysine 56 connects, through hydrogen-bonds, the [4Fe-4S] center to one of the pyranopterin ligands of the Mo-cofactor | Cereibacter sphaeroides f. sp. denitrificans | |
[4Fe-4S]-center | a [4Fe-4S] cluster and two c-type hemes form an intramolecular electron transfer chain that deliver electrons to the active site. Residue lysine 56 connects, through hydrogen-bonds, the [4Fe-4S] center to one of the pyranopterin ligands of the Mo-cofactor | Cereibacter sphaeroides f. sp. denitrificans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
155 | - |
nitrate | mutant K56H, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans | |
250 | - |
nitrate | mutant K56M, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans | |
360 | - |
nitrate | wild-type, 25°C, pH 6 | Cereibacter sphaeroides f. sp. denitrificans |