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Literature summary for 1.8.99.B2 extracted from
- TsdC, a unique lipoprotein from Wolinella succinogenes that enhances tetrathionate reductase activity of TsdA (2017), FEMS Microbiol. Lett., 364, fnx003 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| TsdC | a unique membrane-associated lipoprotein from Wolinella succinogenes, enhances tetrathionate reductase activity of enzyme TsdA. This effect is much stronger in the tetrathionate-reducing than in the thiosulfate-oxidizing direction. The TsdAC complex predominantly acts as a tetrathionate reductase in vivo | Wolinella succinogenes |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tsdA, recombinant expression of Strep-tagged enzyme in Escherichia coli strains BL21(DE3) and C43(DE3), coexpression with Strep-tagged Wolinella succinogenes TsdC | Wolinella succinogenes |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | TsdC from Wolinella succinogenes mediates membrane attachment of TsdA | Wolinella succinogenes | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
about, recombinant enzyme, gel filtration | Wolinella succinogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Wolinella succinogenes | the TsdAC complex predominantly acts as a tetrathionate reductase in vivo | ? | - |
- |
 |
| tetrathionate + reduced electron acceptor | Wolinella succinogenes | - |
2 thiosulfate + oxidized electron acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wolinella succinogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Strep-tagged enzyme TsdA 41fold from Escherichia coli strains BL21(DE3) and C43(DE3) by membrane solubilization, affinity chromatography, desalting gel filtration, and ultrafiltration recombinant Strep-tagged TsdC by two steps of ultracentrifugation at 100000 x g, followed by affinity chromatography | Wolinella succinogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47 | - |
recombinant membranes from the Escherichia coli strain producing the WsTsdAC, pH 5.5, 39°C | Wolinella succinogenes |
| 1975 | - |
purified recombinant TsdAC, pH 5.5, 39°C | Wolinella succinogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the TsdAC complex predominantly acts as a tetrathionate reductase in vivo | Wolinella succinogenes | ? | - |
- |
|
| additional information | recombinant WsTsdA purified from Escherichia coli catalyzes both thiosulfate oxidation and tetrathionate reduction. Activity measurement by performing thiosulfate-dependent ferricyanide reduction | Wolinella succinogenes | ? | - |
- |
|
| tetrathionate + reduced electron acceptor | - |
Wolinella succinogenes | 2 thiosulfate + oxidized electron acceptor | - |
? | |
| tetrathionate + reduced methyl viologen | - |
Wolinella succinogenes | 2 thiosulfate + oxidized methyl viologen | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40028, TsdA diheme cytochrome c with hemes, lacking the 21-aa signal peptide, sequence calculation | Wolinella succinogenes |
| More | the tsdA-like gene from Wolinella succinogenes encodes a periplasmic diheme cytochrome c with hemes, lacking the 21-aa signal peptide. Two Cys-X-X-Cys-His heme c binding motifs are present in the sequence. The active site heme (heme 1) is characterized by an unusual His/Cys axial ligation in TsdA enzymes | Wolinella succinogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TsdA | - |
Wolinella succinogenes |
| WsTsdA | - |
Wolinella succinogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 39 | - |
assay at | Wolinella succinogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Wolinella succinogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | the enzyme TSdA is a periplasmic diheme cytochrome c with hemes. Two Cys-X-X-Cys-His heme c binding motifs are present in the sequence. The active site heme (heme 1) is characterized by an unusual His/Cys axial ligation in TsdA enzymes | Wolinella succinogenes | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | TsdA is closely associated with the unprecedented lipoprotein TsdC encoded immediately downstream of tsdA in the same direction of transcription. TsdA from Wolinella succinogenes is a periplasmic diheme cytochrome c with hemes, lacking the 21-aa signal peptide. Two Cys-X-X-Cys-His heme c binding motifs are present in the sequence. The active site heme (heme 1) is characterized by an unusual His/Cys axial ligation in TsdA enzymes | Wolinella succinogenes |
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