Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 0.1 M NaCl, 2.0 M ammonium sulfate and 15% (v/v) glycerol, at 4°C | Desulfovibrio vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
1 * 75000 + 1 * ?, X-ray crystallography | Desulfovibrio vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio vulgaris | B8PS61 and B8DS74 | alpha subunit and beta subunit | - |
Desulfovibrio vulgaris Miyazaki F | B8PS61 and B8DS74 | alpha subunit and beta subunit | - |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl 650S column chromatography, Sephacryl S-200 gel filtration, and Superdex 200 gel filtration | Desulfovibrio vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl sulfate + reduced acceptor | - |
Desulfovibrio vulgaris | AMP + sulfite + acceptor | - |
? | |
adenylyl sulfate + reduced acceptor | - |
Desulfovibrio vulgaris Miyazaki F | AMP + sulfite + acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 75000 + 1 * ?, X-ray crystallography | Desulfovibrio vulgaris |
Synonyms | Comment | Organism |
---|---|---|
adenylylsulfate reductase | - |
Desulfovibrio vulgaris |
APS reductase | - |
Desulfovibrio vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | contains two 4Fe-4S clusters in the beta-subunit | Desulfovibrio vulgaris | |
FAD | contains a noncovalently bound FAD | Desulfovibrio vulgaris |