Literature summary for 1.8.98.7 extracted from

Benjdia, A.; Leprince, J.; Guillot, A.; Vaudry, H.; Rabot, S.; Berteau, O.
Anaerobic sulfatase-maturating enzymes: radical SAM enzymes able to catalyze in vitro sulfatase post-translational modification (2007), J. Am. Chem. Soc., 129, 3462-3463.

Search for more literature for 1.8.98.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Clostridium perfringens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
[4Fe-4S] cluster	-	Clostridium perfringens

Organism

Organism	UniProt	Comment	Textmining
Clostridium perfringens	Q0TTH1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA Sepharose and Superdex S200HR chromatography	Clostridium perfringens

Storage Stability

Storage Stability	Organism
-80°C, 0.1 M Tris-HCl buffer, pH 7, 150 mM KCl and 5 mM DTT, anaerobic conditions	Clostridium perfringens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ac-FENAYTAVPSCIASRASILTMSQ-NH2 + S-adenosyl-L-methionine + dithionite	-	Clostridium perfringens	Ac-FENAYTAVPS-3-oxo-L-Ala-IASRASILTMSQ-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide	-	?
additional information	the enzyme converts a cysteine residue of arylsulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases	Clostridium perfringens	?	-	?

Synonyms

Synonyms	Comment	Organism
anaerobic sulfatase-maturating enzyme	-	Clostridium perfringens
anSME	-	Clostridium perfringens

General Information

General Information	Comment	Organism
metabolism	involved in the maturation of sulfatases	Clostridium perfringens

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Release 2023.1 (January 2023)