BRENDA - Enzyme Database
show all sequences of 1.8.7.3

A ferredoxin- and F420H2-dependent, electron-bifurcating, heterodisulfide reductase with homologs in the domains Bacteria and Archaea

Yan, Z.; Wang, M.; Ferry, J.G.; mBio 8, e02285-16 (2017)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
CoM-S-S-CoB
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.1
-
CoB
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.2
-
CoB
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.2
-
CoM
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.4
-
CoM
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
required
Methanosarcina acetivorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
Methanosarcina acetivorans
-
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
-
r
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
Methanosarcina acetivorans
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Methanosarcina acetivorans
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Methanosarcina acetivorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
743210
Methanosarcina acetivorans
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
-
-
r
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
743210
Methanosarcina acetivorans
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 18000, subunit HdrC2, SDS-PAGE; x * 33000, subunit HdrB2, SDS-PAGE
Methanosarcina acetivorans
Synonyms
Synonyms
Commentary
Organism
HdrB2
subunit
Methanosarcina acetivorans
HdrB2C2
subunit
Methanosarcina acetivorans
Cofactor
Cofactor
Commentary
Organism
Structure
[4Fe-4S]-center
-
Methanosarcina acetivorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[4Fe-4S]-center
-
Methanosarcina acetivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
CoM-S-S-CoB
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.1
-
CoB
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.2
-
CoB
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.2
-
CoM
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.4
-
CoM
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
required
Methanosarcina acetivorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
Methanosarcina acetivorans
-
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
-
r
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
Methanosarcina acetivorans
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanosarcina acetivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
743210
Methanosarcina acetivorans
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
-
-
r
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
743210
Methanosarcina acetivorans
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 18000, subunit HdrC2, SDS-PAGE; x * 33000, subunit HdrB2, SDS-PAGE
Methanosarcina acetivorans
Other publictions for EC 1.8.7.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743210
Yan
A ferredoxin- and F420H2-depe ...
Methanosarcina acetivorans
mBio
8
e02285-16
2017
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-
1
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-
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5
-
1
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2
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1
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1
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2
1
2
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1
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1
1
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-
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5
-
1
-
2
-
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-
1
-
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2
1
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-
742500
Kroeninger
Evidence for the involvement ...
Methanomassiliicoccus luminyensis, Methanomassiliicoccus luminyensis DSM 25720
FEBS J.
283
472-483
2016
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-
-
-
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1
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2
-
2
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4
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2
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4
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742382
Ramos
The FlxABCD-HdrABC proteins c ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris ATCC 29579
Environ. Microbiol.
17
2288-2305
2015
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1
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4
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2
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1
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4
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1
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4
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745383
Fielding
Advanced electron paramagneti ...
Methanothermobacter marburgensis
J. Biol. Inorg. Chem.
18
905-915
2013
-
-
-
-
-
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-
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1
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1
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1
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1
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1
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744972
Mangold
Sulfur metabolism in the extr ...
Acidithiobacillus caldus
Front. Microbiol.
10
17
2011
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1
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1
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1
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745797
Buan
Methanogenesis by Methanosarc ...
Methanosarcina acetivorans
Mol. Microbiol.
75
843-853
2010
-
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1
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1
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1
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1
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4
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1
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744307
Hamann
A cysteine-rich CCG domain co ...
Methanothermobacter marburgensis
Biochemistry
46
12875-12885
2007
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1
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