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Literature summary for 1.8.5.9 extracted from

  • McMahon, R.; Ireland, P.; Sarovich, D.; Petit, G.; Jenkins, C.; Sarkar-Tyson, M.; Currie, B.; Martin, J.
    Virulence of the melioidosis pathogen Burkholderia pseudomallei requires the oxidoreductase membrane protein DsbB (2018), Infect. Immun., 86, e00938-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 cells Burkholderia pseudomallei

Protein Variants

Protein Variants Comment Organism
C41S/C44S/C102S/C130S inactive Burkholderia pseudomallei
R118H missense mutation Burkholderia pseudomallei
T73I missense mutation Burkholderia pseudomallei
V16M missense mutation Burkholderia pseudomallei
V56A missense mutation Burkholderia pseudomallei

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Burkholderia pseudomallei 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[DsbA protein] with reduced L-cysteine residues + ubiquinone-1 Burkholderia pseudomallei overall reaction [DsbA protein] carrying a disulfide bond + ubiquinol-1
-
 ?
[DsbA protein] with reduced L-cysteine residues + ubiquinone-1 Burkholderia pseudomallei K96243 overall reaction [DsbA protein] carrying a disulfide bond + ubiquinol-1
-
 ?
[DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond Burkholderia pseudomallei
-
 [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
[DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond Burkholderia pseudomallei K96243
-
 [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
[DsbB protein] with reduced L-cysteine residues + ubiquinone-1 Burkholderia pseudomallei
-
 [DsbB protein] carrying a disulfide bond + ubiquinol-1
-
 ?
[DsbB protein] with reduced L-cysteine residues + ubiquinone-1 Burkholderia pseudomallei K96243
-
 [DsbB protein] carrying a disulfide bond + ubiquinol-1
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia pseudomallei Q63RY4
-
-
Burkholderia pseudomallei K96243 Q63RY4
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel affinity column chromatography and Superdex S200 gel filtration Burkholderia pseudomallei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[DsbA protein] with reduced L-cysteine residues + ubiquinone-1 overall reaction Burkholderia pseudomallei [DsbA protein] carrying a disulfide bond + ubiquinol-1
-
 ?
[DsbA protein] with reduced L-cysteine residues + ubiquinone-1 overall reaction Burkholderia pseudomallei K96243 [DsbA protein] carrying a disulfide bond + ubiquinol-1
-
 ?
[DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond
-
 Burkholderia pseudomallei [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
[DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond
-
 Burkholderia pseudomallei K96243 [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
[DsbB protein] with reduced L-cysteine residues + ubiquinone-1
-
 Burkholderia pseudomallei [DsbB protein] carrying a disulfide bond + ubiquinol-1
-
 ?
[DsbB protein] with reduced L-cysteine residues + ubiquinone-1
-
 Burkholderia pseudomallei K96243 [DsbB protein] carrying a disulfide bond + ubiquinol-1
-
 ?

Synonyms

Synonyms Comment Organism
DsbB
-
 Burkholderia pseudomallei
membrane protein disulfide bond protein B
-
 Burkholderia pseudomallei

General Information

General Information Comment Organism
physiological function the enzyme is required for virulence in Burkholderia pseudomallei Burkholderia pseudomallei