Literature summary for 1.8.5.9 extracted from

Malojcic, G.; Owen, R.; Grimshaw, J.; Glockshuber, R.
Preparation and structure of the charge-transfer intermediate of the transmembrane redox catalyst DsbB (2008), FEBS Lett., 582, 3301-3307 .

Search for more literature for 1.8.5.9

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme complexed with ubiquinone Q8 and DsbA, sitting drop vapor diffusion method, using 30% (w/v) PEG 550MME, 0.3 M ammonium formate and 0.05 M Tris/HCl, pH 8.9	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
C130S	inactive	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inner membrane	-	Escherichia coli	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[DsbA protein] with reduced L-cysteine residues + ubiquinone Q8	Escherichia coli	overall reaction	[DsbA protein] carrying a disulfide bond + ubiquinol Q8	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A6M2	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 75 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[DsbA protein] with reduced L-cysteine residues + ubiquinone Q8	overall reaction	Escherichia coli	[DsbA protein] carrying a disulfide bond + ubiquinol Q8	-	?

Subunits

Subunits	Comment	Organism
?	x * 21000, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
DsbB	-	Escherichia coli

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Release 2023.1 (January 2023)