Literature summary for 1.8.5.1 extracted from

Pandey, P.; Achary, V.M.; Kalasamudramu, V.; Mahanty, S.; Reddy, G.M.; Reddy, M.K.
Molecular and biochemical characterization of dehydroascorbate reductase from a stress adapted C4 plant, pearl millet [Pennisetum glaucum (L.) R. Br] (2014), Plant Cell Rep., 33, 435-445 .

Search for more literature for 1.8.5.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rossetta pLysS cells	Cenchrus americanus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04167	-	glutathione	at pH 7.0 and 40°C	Cenchrus americanus
0.1334	-	dehydroascorbate	at pH 7.0 and 40°C	Cenchrus americanus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
glutathione + dehydroascorbate	Cenchrus americanus	-	glutathione disulfide + ascorbate	-	?

Organism

Organism	UniProt	Comment	Textmining
Cenchrus americanus	U5XYA0	pearl millet	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Cenchrus americanus

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Cenchrus americanus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + dehydroascorbate	-	Cenchrus americanus	glutathione disulfide + ascorbate	-	?

Subunits

Subunits	Comment	Organism
?	x * 26000, SDS-PAGE	Cenchrus americanus
?	x * 23400, calculated from amino acid sequence	Cenchrus americanus

Synonyms

Synonyms	Comment	Organism
dehydroascorbate reductase	-	Cenchrus americanus
DHAR	-	Cenchrus americanus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Cenchrus americanus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	50	more than 80% activity between 30 and 50°C	Cenchrus americanus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Cenchrus americanus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8	more than 50% activity between pH 6.5 and 8.0	Cenchrus americanus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Cenchrus americanus	calculated from amino acid sequence	-	6

Expression

Organism	Comment	Expression
Cenchrus americanus	the enzyme expression is down-regulated under salinity stress (250 mM NaCl)	down
Cenchrus americanus	the enzyme expression is up-regulated in response to high temperature (45°C)	up

General Information

General Information	Comment	Organism
physiological function	the enzyme plays a protective role under oxidative and other abiotic stress conditions	Cenchrus americanus

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Release 2023.1 (January 2023)