Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ipomoea batatas |
General Stability | Organism |
---|---|
resistant to digestion by trypsin and chymotrypsin even at a high enzyme/substrate (w/w) ratio of 1:10 | Ipomoea batatas |
slight decrease in activity with increasing concentration of imidazole to 0.8 M | Ipomoea batatas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
dehydroascorbate | 25°C | Ipomoea batatas | |
2.38 | - |
glutathione | 25°C | Ipomoea batatas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 25000, SDS-PAGE | Ipomoea batatas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | D2CGD4 | (L.) Lam | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Ipomoea batatas |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Ipomoea batatas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | - |
Ipomoea batatas | glutathione disulfide + ascorbate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, SDS-PAGE | Ipomoea batatas |
Synonyms | Comment | Organism |
---|---|---|
dehydroascorbate reductase | - |
Ipomoea batatas |
DHAR | - |
Ipomoea batatas |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life: 10.1 min | Ipomoea batatas |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100.6 | - |
glutathione | 25°C | Ipomoea batatas | |
368.2 | - |
dehydroascorbate | 25°C | Ipomoea batatas |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | stable | Ipomoea batatas |