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show all sequences of 1.8.4.13

Methionine sulfoxide reductases: history and cellular role in protecting against oxidative damage

Weissbach, H.; Resnick, L.; Brot, N.; Biochim. Biophys. Acta 1703, 203-212 (2005)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
enzyme can be useful in the development and action of anti-cancer and anti-inflammation drugs
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
enzyme form Mem-R,S-Msr
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-methionine (S)-sulfoxide + NADPH + H+
Escherichia coli
membrane-bound enzyme form Mem-R,S-Msr
L-methionine + NADP+ + H2O
-
-
?
additional information
Escherichia coli
the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions
?
-
-
-
sulindac + NADPH
Escherichia coli
activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr
sulindac sulfide + NADP+ + H2O
activated drug which inhibits cyclooxygenase 1 and 2 and exhibits anti-inflammatory activity
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
; isozyme Mem-R,S-Msr, bifunctional enzyme
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00009
-
enzyme form Mem-R,S-Msr, substrate sulindac
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-methionine (S)-sulfoxide + NADPH + H+
membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
L-methionine + NADP+ + H2O
-
-
-
?
L-methionine sulfoxide enkephalin + NADPH
membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
L-methionine enkephalin + NADP+ + H2O
-
-
-
?
L-methionine-(S)-S-oxide + thioredoxin
-
658212
Escherichia coli
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
additional information
the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions
658212
Escherichia coli
?
-
-
-
-
additional information
substrate specificity, overview, Mem-R,S-Msr can also catalyze the reduction of L-methionine (R)-sulfoxide, EC 1.8.4.14, enzyme reduces oxidized methionine residues of the ribosomal protein L12, which becomes reversibly inactivated and forms monomers instead of dimers upon oxidation
658212
Escherichia coli
?
-
-
-
-
additional information
no activity with peptide-L-methionine-(S)-S-oxide
658212
Escherichia coli
?
-
-
-
-
N-acetyl-L-methionine (S)-sulfoxide + thioredoxin
membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
-
-
?
sulindac + NADPH
activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
sulindac sulfide + NADP+ + H2O
activated drug which inhibits cyclooxygenase 1 and 2 and exhibits anti-inflammatory activity
-
-
?
sulindac + NADPH
activity with membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
sulindac sulfide + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with DTT as cofactor by membrane-bound enzyme form Mem-R,S-Msr
Escherichia coli
NADPH
membrane-bound enzyme form Mem-R,S-Msr
Escherichia coli
Application (protein specific)
Application
Commentary
Organism
synthesis
enzyme can be useful in the development and action of anti-cancer and anti-inflammation drugs
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with DTT as cofactor by membrane-bound enzyme form Mem-R,S-Msr
Escherichia coli
NADPH
membrane-bound enzyme form Mem-R,S-Msr
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
enzyme form Mem-R,S-Msr
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-methionine (S)-sulfoxide + NADPH + H+
Escherichia coli
membrane-bound enzyme form Mem-R,S-Msr
L-methionine + NADP+ + H2O
-
-
?
additional information
Escherichia coli
the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions
?
-
-
-
sulindac + NADPH
Escherichia coli
activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr
sulindac sulfide + NADP+ + H2O
activated drug which inhibits cyclooxygenase 1 and 2 and exhibits anti-inflammatory activity
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00009
-
enzyme form Mem-R,S-Msr, substrate sulindac
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-methionine (S)-sulfoxide + NADPH + H+
membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
L-methionine + NADP+ + H2O
-
-
-
?
L-methionine sulfoxide enkephalin + NADPH
membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
L-methionine enkephalin + NADP+ + H2O
-
-
-
?
L-methionine-(S)-S-oxide + thioredoxin
-
658212
Escherichia coli
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
additional information
the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions
658212
Escherichia coli
?
-
-
-
-
additional information
substrate specificity, overview, Mem-R,S-Msr can also catalyze the reduction of L-methionine (R)-sulfoxide, EC 1.8.4.14, enzyme reduces oxidized methionine residues of the ribosomal protein L12, which becomes reversibly inactivated and forms monomers instead of dimers upon oxidation
658212
Escherichia coli
?
-
-
-
-
additional information
no activity with peptide-L-methionine-(S)-S-oxide
658212
Escherichia coli
?
-
-
-
-
N-acetyl-L-methionine (S)-sulfoxide + thioredoxin
membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
-
-
?
sulindac + NADPH
activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
sulindac sulfide + NADP+ + H2O
activated drug which inhibits cyclooxygenase 1 and 2 and exhibits anti-inflammatory activity
-
-
?
sulindac + NADPH
activity with membrane-bound enzyme form Mem-R,S-Msr
658212
Escherichia coli
sulindac sulfide + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.8.4.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
658212
Weissbach
Methionine sulfoxide reductase ...
Escherichia coli
Biochim. Biophys. Acta
1703
203-212
2005
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1
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1
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3
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1
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1
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9
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2
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1
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2
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1
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3
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1
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9
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393068
Ejiri
The purification of methionine ...
Escherichia coli, Escherichia coli B
Anal. Biochem.
102
393-398
1980
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1
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1
1
1
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2
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1
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1
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3
1
1
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1
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1
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1
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1
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1
1
1
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1
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1
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3
1
1
-
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1
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393071
Ejiri
Reduction of methionine sulfox ...
Escherichia coli K-12 2276, Escherichia coli K-12
J. Bacteriol.
139
161-164
1979
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2
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2
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2
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1
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4
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1
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1
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1
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1
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2
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2
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1
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4
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1
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1
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393070
Black
-
A specific enzymatic reduction ...
Saccharomyces cerevisiae
J. Biol. Chem.
235
2910-2916
1960
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7
1
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2
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1
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1
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4
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1
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1
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1
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1
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7
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1
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2
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1
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4
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1
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1
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