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Literature summary for 1.8.4.12 extracted from

  • Arias, D.G.; Cabeza, M.S.; Echarren, M.L.; Faral-Tello, P.; Iglesias, A.A.; Robello, C.; Guerrero, S.A.
    On the functionality of a methionine sulfoxide reductase B from Trypanosoma cruzi (2020), Free Radic. Biol. Med., 158, 96-114 .
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
C128S inactive mutant enzyme Trypanosoma cruzi
C72S similar catalytic efficiency (regarding wild-type protein) for the reduction of N-acetyl-L-methionine-(R)-S-oxide. About 7fold less efficient for tryparedoxin I oxidation than the wild-type enzyme. The mutant enzyme does not exhibit changes in electrophoretic motility respect to wild-type enzyme Trypanosoma cruzi
C72S/C74S similar catalytic efficiency (regarding wild-type protein) for the reduction of N-acetyl-L-methionine-(R)-S-oxide. The mutant enzyme is unable to use Trypanosoma cruzi tryparedoxin or Trypanosoma cruzi thioredoxin as reducing substrates, being able to use trypanothione disulfide as a direct reducing substrate. The double mutation allows the protein to use glutathione as a reductant Trypanosoma cruzi
C74S similar catalytic efficiency (regarding wild-type protein) for the reduction of N-acetyl-L-methionine-(R)-S-oxide. About 2fold less efficient for tryparedoxin I oxidation than the wild-type enzyme. The mutant enzyme does not exhibit changes in electrophoretic motility respect to wild-type enzyme Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C72S Trypanosoma cruzi
0.0019
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C74S Trypanosoma cruzi
0.0021
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Trypanosoma cruzi
0.0055
-
 thioredoxin pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Trypanosoma cruzi
0.043
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: trypanothione, mutant enzyme C72S/C74S Trypanosoma cruzi
0.045
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I, mutant enzyme C72S Trypanosoma cruzi
0.047
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I, mutant enzyme C74S Trypanosoma cruzi
0.2
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I Trypanosoma cruzi
0.204
-
 trypanothione disulfide pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C72S/C74S Trypanosoma cruzi
1
-
 L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Trypanosoma cruzi 5829
-
mitochondrion
-
 Trypanosoma cruzi 5739
-

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi V5BL87
-
-
Trypanosoma cruzi Dm28c V5BL87
-
-

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine-(R)-S-oxide + tryparedoxin I
-
 Trypanosoma cruzi L-methionine + tryparedoxin I disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + tryparedoxin I
-
 Trypanosoma cruzi Dm28c L-methionine + tryparedoxin I disulfide + H2O
-
 ?
additional information no activity with glutathione Trypanosoma cruzi ?
-
-
additional information no activity with glutathione Trypanosoma cruzi Dm28c ?
-
-
N-acetyl-L-methionine-(R)-S-oxide + thioredoxin
-
 Trypanosoma cruzi N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + thioredoxin
-
 Trypanosoma cruzi Dm28c N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + tryparedoxin I
-
 Trypanosoma cruzi N-acetyl-L-methionine + tryparedoxin I disulfide + H2O
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + tryparedoxin I
-
 Trypanosoma cruzi Dm28c N-acetyl-L-methionine + tryparedoxin I disulfide + H2O
-
 ?
trypanothione disulfide + NADPH + H+
-
 Trypanosoma cruzi trypanothione + NADP+
-
 ?
trypanothione disulfide + NADPH + H+
-
 Trypanosoma cruzi Dm28c trypanothione + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase B
-
 Trypanosoma cruzi
MsrB
-
 Trypanosoma cruzi
TCDM_06423
-
 Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00052
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I, mutant enzyme C72S Trypanosoma cruzi
0.00067
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C72S Trypanosoma cruzi
0.0016
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I, mutant enzyme C74S Trypanosoma cruzi
0.002
-
 L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I, wild-type enzyme Trypanosoma cruzi
0.0023
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: trypanothione, mutant enzyme C72S/C74S Trypanosoma cruzi
0.0025
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C74S Trypanosoma cruzi
0.0048
-
 trypanothione disulfide pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C72S/C74S Trypanosoma cruzi
0.0048
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I, wild-type enzyme Trypanosoma cruzi
0.0056
-
 thioredoxin pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, wild-type enzyme Trypanosoma cruzi
0.00583
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, wild-type enzyme Trypanosoma cruzi

General Information

General Information Comment Organism
metabolism epimastigotes overexpressing the enzyme (MSRB) exhibit inhibition of the metacyclogenesis process Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
 L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I Trypanosoma cruzi
0.011
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I, mutant enzyme C72S Trypanosoma cruzi
0.024
-
 trypanothione disulfide pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C72S/C74S Trypanosoma cruzi
0.024
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I Trypanosoma cruzi
0.034
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: tryparedoxin I, mutant enzyme C74S Trypanosoma cruzi
0.054
-
 N-acetyl-L-methionine-(R)-S-oxide pH 7.5, 30°C, cosubstrate: trypanothione, mutant enzyme C72S/C74S Trypanosoma cruzi
0.417
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C72S Trypanosoma cruzi
1.03
-
 thioredoxin pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Trypanosoma cruzi
1.307
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C74S Trypanosoma cruzi
2.778
-
 tryparedoxin I pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Trypanosoma cruzi