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Literature summary for 1.8.4.11 extracted from

  • Peng, L.; Wen, Y.; Chen, Y.; Yuan, Z.; Zhou, Y.; Cheng, X.; Chen, Y.; Yang, J.
    Biocatalytic preparation of chiral sulfoxides through asymmetric reductive resolution by methionine sulfoxide reductase A (2018), ChemCatChem, 10, 3284-3290 .
No PubMed abstract available
Search for more literature for 1.8.4.11

Application

Application Comment Organism
synthesis the enzyme can effectively accomplish the preparation of (R)-sulfoxides with approximately 50% yield and 94-99% enantiomeric excess through asymmetric reductive resolution of racemic sulfoxide. With the establishment of the enzyme regeneration system, the initial substrate concentration can be increased 40-100times. The (R)-sulfoxides are obtained with high enantioselectivity under the substrate concentration up to 200 mm (approximately 32 g/L), representing a quite high substrate concentration in biocatalytic preparation of chiral sulfoxides. This system shows fairly good activity and enantioselectivity towards a series of ortho- and para-substituted phenyl methyl sulfoxides under high substrate concentration Pseudomonas monteilii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Pseudomonas monteilii

Organic Solvent Stability

Organic Solvent Comment Organism
1,4-dioxane the enzyme retains more than 75% activity at a concentration of 8% Pseudomonas monteilii
2-propanol the enzyme retains more than 75% activity at a concentration of 8% Pseudomonas monteilii
Ethanol the enzyme retains more than 75% activity at a concentration of 8% Pseudomonas monteilii
Methanol the enzyme retains more than 75% activity at a concentration of 8% Pseudomonas monteilii

Organism

Organism UniProt Comment Textmining
Pseudomonas monteilii A0A6B9VRC3
-
-
Pseudomonas monteilii CCTCC M2013683 A0A6B9VRC3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas monteilii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
racemic (methanesulfinyl)benzene + dithiothreitol
-
 Pseudomonas monteilii (methylsulfanyl)benzene + [(R)-methanesulfinyl]benzene + dithiothreitol disulfide + H2O
-
 ?
racemic (methanesulfinyl)benzene + dithiothreitol
-
 Pseudomonas monteilii CCTCC M2013683 (methylsulfanyl)benzene + [(R)-methanesulfinyl]benzene + dithiothreitol disulfide + H2O
-
 ?
racemic 1-bromo-4-(methanesulfinyl)benzene + dithiothreitol
-
 Pseudomonas monteilii 1-bromo-4-(methylsulfanyl)benzene + 1-bromo-4-[(R)-methanesulfinyl]benzene + dithiothreitol disulfide + H2O
-
 ?
racemic 1-bromo-4-(methanesulfinyl)benzene + dithiothreitol
-
 Pseudomonas monteilii CCTCC M2013683 1-bromo-4-(methylsulfanyl)benzene + 1-bromo-4-[(R)-methanesulfinyl]benzene + dithiothreitol disulfide + H2O
-
 ?
racemic 1-fluoro-4-(methanesulfinyl)benzene + dithiothreitol
-
 Pseudomonas monteilii 1-fluoro-4-(methylsulfanyl)benzene + 1-fluoro-4-[(R)-methanesulfinyl]benzene + dithiothreitol disulfide + H2O
-
 ?
racemic 1-fluoro-4-(methanesulfinyl)benzene + dithiothreitol
-
 Pseudomonas monteilii CCTCC M2013683 1-fluoro-4-(methylsulfanyl)benzene + 1-fluoro-4-[(R)-methanesulfinyl]benzene + dithiothreitol disulfide + H2O
-
 ?
racemic 1-methyl-4-(methanesulfinyl)benzene + dithiothreitol
-
 Pseudomonas monteilii 1-methyl-4-(methylsulfanyl)benzene + 1-methyl-4-[(R)-methanesulfinyl]benzene + dithiothreitol disulfide + H2O
-
 ?
racemic 1-methyl-4-(methanesulfinyl)benzene + dithiothreitol
-
 Pseudomonas monteilii CCTCC M2013683 1-methyl-4-(methylsulfanyl)benzene + 1-methyl-4-[(R)-methanesulfinyl]benzene + dithiothreitol disulfide + H2O
-
 ?
racemic 2-(methanesulfinyl)aniline + dithiothreitol
-
 Pseudomonas monteilii 2-(methylsulfanyl)aniline + 2-[(R)-methanesulfinyl]aniline + dithiothreitol disulfide + H2O
-
 ?
racemic 2-(methanesulfinyl)aniline + dithiothreitol
-
 Pseudomonas monteilii CCTCC M2013683 2-(methylsulfanyl)aniline + 2-[(R)-methanesulfinyl]aniline + dithiothreitol disulfide + H2O
-
 ?
racemic 2-(methanesulfinyl)phenol + dithiothreitol
-
 Pseudomonas monteilii 2-(methylsulfanyl)phenol + 2-[(R)-methanesulfinyl]phenol + dithiothreitol disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MsrA
-
 Pseudomonas monteilii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas monteilii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 the enzyme retains almost 80% of its activity between 20 and 40°C. Less than 1% of activity remains at 55°C Pseudomonas monteilii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas monteilii

pH Range

pH Minimum pH Maximum Comment Organism
6 10 the enzyme has more than 60% of activity between pH 7.0 and pH 10.0 Pseudomonas monteilii