Cloned (Comment) | Organism |
---|---|
complementation of the msrA1/msrA2 double mutant with the msr1 construct, promotor exchange between msrA1 and msrA2 | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a msrA2 knockout mutant, a msrA1 knockout mutant, and a msrA1/msrA2 double knockout mutant, the msrA2 mutant strain and the msrA1/msrA2 double mutant strain show H2O2 tolerance like the wild-type parent strain, but the double mutant strain complemented by msrA2 is H2O2 susceptible, effects of H2O2 or oxidative stress are related to the promotors, overview | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | Staphylococcus aureus | MsrAs are specific for the (S)-form of the substrate | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | Staphylococcus aureus | the MsrA1/MsrB system is physiologically more significant in Staphylococcus aureus than MsrA2 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
strains RN450 and BB270, and derivatives, 3 genes msrA1, msrA2, and msrA3 encoding MsrA enzyme forms | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
69% of wild-type strain RB450 MsrA activity belongs to MsrA1, 8% to MsrA2, and 23% to MsrA3 | Staphylococcus aureus |
0.00006 | - |
msrA1/msrA2 double knockout strain RN450 | Staphylococcus aureus |
0.00006 | - |
msrA1/msrA2 double knockout strain RN450, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
0.00008 | - |
msrA1 knockout strain RN450 | Staphylococcus aureus |
0.00008 | - |
msrA1 knockout strain RN450, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
0.00024 | - |
msrA2 knockout strain RN450 | Staphylococcus aureus |
0.00024 | - |
msrA2 knockout strain RN450, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
0.00026 | - |
wild-type strain RN450 | Staphylococcus aureus |
0.00026 | - |
wild-type strain RN450, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | MsrAs are specific for the (S)-form of the substrate | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | the MsrA1/MsrB system is physiologically more significant in Staphylococcus aureus than MsrA2 | Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Staphylococcus aureus |