Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta(DE3)pLysS strain | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D24A | the mutant enzyme oxidizes GSH and gamma-glutamylcysteine at much lower rates than the wild-type enzyme | Saccharomyces cerevisiae |
N131A | the mutant enzyme oxidizes GSH and gamma-glutamylcysteine at much lower rates than the wild-type enzyme | Saccharomyces cerevisiae |
N34A | enhancement of catalytic activity for GSH, whereas the catalytic activity for gamma-glutamylcysteine remains unchanged. The mutant enzyme shows slightly decreased maximum temperatures at 55°C (compared to 60°C for the wild-type enzyme) | Saccharomyces cerevisiae |
N34Q | the mutant enzyme oxidizes GSH more efficiently (201%) than the wild-type enzyme | Saccharomyces cerevisiae |
P129A | the mutant enzyme oxidizes GSH and gamma-glutamylcysteine at much lower rates than the wild-type enzyme | Saccharomyces cerevisiae |
S32A | the mutant enzyme oxidizes GSH more efficiently (169%) than the wild-type enzyme. About 1.5fold increase in GSSG production compared to that of the parental ERV1 gene | Saccharomyces cerevisiae |
S32A/N34A | the mutant enzyme oxidizes GSH more efficiently (240%) than the wild-type enzyme and shows comparable activity for gamma-glutamylcysteine (96%). The mutant enzyme shows slightly decreased maximum temperatures at 55°C (compared to 60°C for the wild-type enzyme) | Saccharomyces cerevisiae |
S32T | the mutant enzyme oxidizes GSH more efficiently (178%) than the wild-type enzyme | Saccharomyces cerevisiae |
S32T/N34A | the mutant enzyme shows almost the same activity for GSH (192%) compared to mutant enzyme S32A, S32T, and N34A, and high activity for gamma-glutamylcysteine (161%). The mutant enzyme shows slightly decreased maximum temperatures at 55°C (compared to 60°C for the wild-type enzyme) | Saccharomyces cerevisiae |
W132A | the mutant enzyme oxidizes GSH and gamma-glutamylcysteine at much lower rates than the wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 3.5 | glutathione | pH 7.0, 30°C, mutant enzyme N34A | Saccharomyces cerevisiae | |
4.4 | - |
glutathione | pH 7.0, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
12 | - |
glutathione | pH 7.0, 30°C, mutant enzyme S32T | Saccharomyces cerevisiae | |
13 | - |
glutathione | pH 7.0, 30°C, mutant enzyme S32A | Saccharomyces cerevisiae | |
40.5 | - |
glutathione | pH 7.0, 30°C, mutant enzyme S32A/N34A | Saccharomyces cerevisiae | |
41.2 | - |
glutathione | pH 7.0, 30°C, mutant enzyme S32T/N34A | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae NBRC 10505 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + O2 | - |
Saccharomyces cerevisiae | glutathione disulfide + H2O2 | - |
? | |
2 glutathione + O2 | - |
Saccharomyces cerevisiae NBRC 10505 | glutathione disulfide + H2O2 | - |
? | |
gamma-glutamylcysteine + O2 | - |
Saccharomyces cerevisiae | ? | - |
? | |
gamma-glutamylcysteine + O2 | - |
Saccharomyces cerevisiae NBRC 10505 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thiol oxidase Erv1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
mutant ezymes N34A, S32A/N34A and S32T/N34A | Saccharomyces cerevisiae |
60 | - |
wild-type enzyme and mutant enzymes S32A and S32R | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
59.1 | - |
T1/2, mutant enzyme S32A/N34A | Saccharomyces cerevisiae |
62 | - |
T1/2, mutant enzyme S32T/N34A | Saccharomyces cerevisiae |
64.1 | - |
T1/2, mutant enzyme N34A | Saccharomyces cerevisiae |
68.8 | - |
T1/2, mutant enzyme S32T | Saccharomyces cerevisiae |
69.3 | - |
T1/2, mutant enzyme S32A | Saccharomyces cerevisiae |
90 | - |
T1/2 is below 90°C, wild-type enzyme | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
glutathione | pH 7.0, 30°C, mutant enzyme S32A | Saccharomyces cerevisiae | |
0.00083 | - |
glutathione | pH 7.0, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.0017 | - |
glutathione | pH 7.0, 30°C, mutant enzyme S32T | Saccharomyces cerevisiae | |
0.0033 | - |
glutathione | pH 7.0, 30°C, mutant enzyme S32T/N34A | Saccharomyces cerevisiae | |
0.0042 | - |
glutathione | pH 7.0, 30°C, mutant enzyme N34A | Saccharomyces cerevisiae | |
0.007 | - |
glutathione | pH 7.0, 30°C, mutant enzyme S32A/N34A | Saccharomyces cerevisiae |