Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TP1004 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nitrite | nitrite inhibits sulfite-dependent cytochrome c reduction at sulfite concentrations ranging from 0.01 to 0.1 mM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | mitochondrial intermembrane space | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + O2 + H2O | Homo sapiens | - |
sulfate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P51687 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + H2O + porcine ferricytochrome c | the nitrite reduction mechanism involves sulfite oxidation, sulfate release and nitrite coordination at molybdenum with protonation-dependent nitric oxide and molybdenum V release. The highest nitric oxide production occurs between 0.01 and 0.0375 mM sulfite, with a dose-dependent inhibition of nitric oxide formation at higher sulfite concentrations | Homo sapiens | nitric oxide + porcine ferrocytochrome c | - |
? | |
sulfite + H2O + porcine ferricyanide | - |
Homo sapiens | sulfate + porcine ferrocyanide | - |
? | |
sulfite + H2O + porcine ferricytochrome c | - |
Homo sapiens | sulfate + porcine ferrocytochrome c | - |
? | |
sulfite + O2 + H2O | - |
Homo sapiens | sulfate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens | |
molybdenum cofactor | - |
Homo sapiens |