Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain TP1000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A208D | the intramolecular electron transfer rate constants at pH 6.0 are decreased by 3 orders of magnitude relative to that of the wild type, the active site structure of the Mo(V) form of A208D is different from that of the wild type | Homo sapiens |
G473A | dimer | Homo sapiens |
G473D | monomer, the Mo(V) active site structure is similar to that of the wild type, and the IET rate constant is only 2.6fold smaller than that of the wild type | Homo sapiens |
G473D/R212A | shows no intramolecular electron transfer rate | Homo sapiens |
G473W | monomer | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00129 | - |
sulfite | wild type enzyme in 20 mM Tris at pH 6.0 | Homo sapiens | |
0.00453 | - |
sulfite | mutant enzyme G473A in 20 mM Tris at pH 6.0 | Homo sapiens | |
0.00825 | - |
sulfite | wild type enzyme in 20 mM Tris at pH 8.5 | Homo sapiens | |
0.0692 | - |
sulfite | mutant enzyme A208D in 20 mM Tris at pH 6.0 | Homo sapiens | |
0.107 | - |
sulfite | mutant enzyme G473A in 20 mM Tris at pH 8.5 | Homo sapiens | |
1.39 | - |
sulfite | mutant enzyme A208D in 20 mM Tris at pH 8.5 | Homo sapiens | |
1.66 | - |
sulfite | mutant enzyme G473D in 20 mM Tris at pH 6.0 | Homo sapiens | |
1.91 | - |
sulfite | mutant enzyme G473W in 20 mM Tris at pH 6.0 | Homo sapiens | |
2.03 | - |
sulfite | mutant enzyme G473W in 20 mM Tris at pH 8.5 | Homo sapiens | |
2.04 | - |
sulfite | mutant enzyme G473D in 20 mM Tris at pH 8.5 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Superdex 200 FPLC column gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + H2O + A | - |
Homo sapiens | SO42- + AH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild type enzyme, mutant enzymes G473A and A208D | Homo sapiens |
monomer | mutant enzymes G473D and G473W | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SO | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
sulfite | mutant enzyme G473D in 20 mM Tris at pH 6.0 | Homo sapiens | |
0.15 | - |
sulfite | mutant enzyme A208D in 20 mM Tris at pH 6.0 | Homo sapiens | |
0.54 | - |
sulfite | mutant enzyme G473D in 20 mM Tris at pH 8.5 | Homo sapiens | |
0.6 | - |
sulfite | mutant enzyme G473W in 20 mM Tris at pH 6.0 | Homo sapiens | |
0.75 | - |
sulfite | mutant enzyme A208D in 20 mM Tris at pH 8.5 | Homo sapiens | |
2.48 | - |
sulfite | mutant enzyme G473W in 20 mM Tris at pH 8.5 | Homo sapiens | |
4.15 | - |
sulfite | mutant enzyme G473A in 20 mM Tris at pH 6.0 | Homo sapiens | |
13.2 | - |
sulfite | wild type enzyme in 20 mM Tris at pH 6.0 | Homo sapiens | |
26.9 | - |
sulfite | wild type enzyme in 20 mM Tris at pH 8.5 | Homo sapiens | |
28.4 | - |
sulfite | mutant enzyme G473A in 20 mM Tris at pH 8.5 | Homo sapiens |