Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 17% inhibition at 5 mM | Rhodotorula sp. | |
2,2'-bipyridyl | not inhibitory at 5 mM | Rhodotorula sp. | |
deoxycholate | - |
Rhodotorula sp. | |
HgCl2 | 100% inhibition at 0.5 mM, 45% inhibition at 0.25 mM and 15% inhibition at 0.1 mM | Rhodotorula sp. | |
N-ethylmaleimide | 12% inhibition at 1 mM | Rhodotorula sp. | |
p-hydroxymercuribenzoate | 50% inhibition at 1 mM, 40% inhibition at 0.5 mM and 10% inhibition at 0.1 mM | Rhodotorula sp. | |
SO32- | - |
Rhodotorula sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
thiosulfate | with native cytochrome c as electron acceptor | Rhodotorula sp. | |
0.16 | - |
thiosulfate | with ferricyanide as electron acceptor | Rhodotorula sp. | |
0.34 | - |
ferricyanide | - |
Rhodotorula sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula sp. | - |
- |
- |
Storage Stability | Organism |
---|---|
-20°C, the activity of cell-free extracts is stable for several days in the presence of 20% v/v glycerol, without glycerol most of the activity is lost after 2 days | Rhodotorula sp. |
-20°C, the activity of whole cells is stable for 3 months | Rhodotorula sp. |
4°C, cell-free extracts lose most of the activity after 2 days even in the presence of glycerol | Rhodotorula sp. |
the activity in cell-free extracts is lost very rapidly after freezing and thawing | Rhodotorula sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + ferricyanide | - |
Rhodotorula sp. | tetrathionate + ferrocyanide | - |
? | |
thiosulfate + oxidized acceptor | ferricyanide or native cytochrome c act as electron acceptors | Rhodotorula sp. | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Rhodotorula sp. | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Rhodotorula sp. | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Rhodotorula sp. | tetrathionate + reduced acceptor | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodotorula sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
30% decrease of activity after 5 min | Rhodotorula sp. |
60 | - |
50% decrease of activity after 7 min | Rhodotorula sp. |
100 | - |
after 2 min the activity is completely destroyed | Rhodotorula sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Rhodotorula sp. |