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Literature summary for 1.8.1.9 extracted from
- Direct cloning of the trxB gene that encodes thioredoxin reductase (1985), J. Bacteriol., 163, 238-242.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of wild-type and mutant enzymes from plasmids | Escherichia coli |
| trxB gene, DNA sequence analysis | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | transposon mutagenesis | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Escherichia coli |
| 65800 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? |  |
| thioredoxin + NADP+ | coupled assay with DTNB | Escherichia coli | thioredoxin disulfide + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, SDS-PAGE | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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