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Literature summary for 1.8.1.8 extracted from
- Purification and characterization of a Bacillus megaterium disulfide reductase specific for disulfides containing pantethine 4',4"-diphosphate (1983), J. Bacteriol., 153, 475-484.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | - |
Priestia megaterium |  |
| N-ethylmaleimide | - |
Priestia megaterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
pantethine 4',4''-diphosphate | - |
Priestia megaterium | |
| 7.4 | - |
CoA-SS-CoA | - |
Priestia megaterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Priestia megaterium |
| 122000 | - |
gel filtration | Priestia megaterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Priestia megaterium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | the enzyme is at a low level in log-phase cells but increases up to 10fold early in the stationary phase and has a similar specific activity in both the mother cell and the forespore compartment. The enzyme activity falls only slowly during spore germination and outgrowth | Priestia megaterium | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is active only on disulfides containing pantethine 4',4"-diphosphate moieties, including pantethine 4',4"-diphosphate, oxidized coenzyme A, and coenzyme A in disulfide linkage to acyl carrier protein | Priestia megaterium | ? | - |
? | |
| NADH + CoA-SS-CoA + H+ | - |
Priestia megaterium | NAD+ + CoA | - |
? | |
| NADH + pantethine 4',4''-diphosphate | - |
Priestia megaterium | NAD+ + 4'-phosphopantetheine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Priestia megaterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH-linked disulfide reductase | - |
Priestia megaterium |
| panthethine 4'4-diphosphate-specific reductase | - |
Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
substrate: pantethine 4',4''-diphosphate | Priestia megaterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
seven times as active in phosphate buffer as in Tris at the same pH | Priestia megaterium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains one molecule of flavin adenine dinucleotide per subunit | Priestia megaterium | |
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