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show all sequences of 1.8.1.16

Characterization of glutathione amide reductase from Chromatium gracile. Identification of a novel thiol peroxidase (Prx/Grx) fueled by glutathione amide redox cycling

Vergauwen, B.; Pauwels, F.; Jacquemotte, F.; Meyer, T.E.; Cusanovich, M.A.; Bartsch, R.G.; van Beeumen, J.J.; J. Biol. Chem. 276, 20890-20897 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Marichromatium gracile
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0132
-
NADH
pH 7.1, 25°C
Marichromatium gracile
0.097
-
glutathione amide disulfide
pH 7.1, 25°C
Marichromatium gracile
1.98
-
NADPH
pH 7.1, 25°C
Marichromatium gracile
6.9
-
glutathione disulfide
pH 7.1, 25°C
Marichromatium gracile
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49028
-
2 * 49028, calculation from sequence
Marichromatium gracile
49030
-
2 * 49030, nanospray ionization mass spectrometry
Marichromatium gracile
98000
-
gel filtration
Marichromatium gracile
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glutathione amide disulfide + NADH + H+
Marichromatium gracile
-
2 glutathione amide + NAD+
-
-
?
glutathione amide disulfide + NADH + H+
Marichromatium gracile DSM 1712
-
2 glutathione amide + NAD+
-
-
?
glutathione amide disulfide + NADPH + H+
Marichromatium gracile
-
2 glutathione amide + NADP+
-
-
?
glutathione amide disulfide + NADPH + H+
Marichromatium gracile DSM 1712
-
2 glutathione amide + NADP+
-
-
?
glutathione disulfide + NADH + H+
Marichromatium gracile
-
2 glutathione + NAD+
-
-
?
glutathione disulfide + NADH + H+
Marichromatium gracile DSM 1712
-
2 glutathione + NAD+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Marichromatium gracile
-
-
-
Purification (Commentary)
Commentary
Organism
-
Marichromatium gracile
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glutathione amide disulfide + NADH + H+
-
698716
Marichromatium gracile
2 glutathione amide + NAD+
-
-
-
?
glutathione amide disulfide + NADH + H+
-
698716
Marichromatium gracile DSM 1712
2 glutathione amide + NAD+
-
-
-
?
glutathione amide disulfide + NADPH + H+
-
698716
Marichromatium gracile
2 glutathione amide + NADP+
-
-
-
?
glutathione amide disulfide + NADPH + H+
-
698716
Marichromatium gracile DSM 1712
2 glutathione amide + NADP+
-
-
-
?
glutathione disulfide + NADH + H+
-
698716
Marichromatium gracile
2 glutathione + NAD+
-
-
-
?
glutathione disulfide + NADH + H+
-
698716
Marichromatium gracile DSM 1712
2 glutathione + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 49028, calculation from sequence; 2 * 49030, nanospray ionization mass spectrometry
Marichromatium gracile
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.14
-
NADPH
pH 7.1, 25°C
Marichromatium gracile
146
-
glutathione disulfide
pH 7.1, 25°C
Marichromatium gracile
186.8
-
NADH
pH 7.1, 25°C
Marichromatium gracile
249.7
-
glutathione amide disulfide
pH 7.1, 25°C
Marichromatium gracile
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
formation of a charge-transfer complex between one of the newly reduced active site thiols and the oxidized FAD
Marichromatium gracile
NADH
preferred cofactor
Marichromatium gracile
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Marichromatium gracile
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
formation of a charge-transfer complex between one of the newly reduced active site thiols and the oxidized FAD
Marichromatium gracile
NADH
preferred cofactor
Marichromatium gracile
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0132
-
NADH
pH 7.1, 25°C
Marichromatium gracile
0.097
-
glutathione amide disulfide
pH 7.1, 25°C
Marichromatium gracile
1.98
-
NADPH
pH 7.1, 25°C
Marichromatium gracile
6.9
-
glutathione disulfide
pH 7.1, 25°C
Marichromatium gracile
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49028
-
2 * 49028, calculation from sequence
Marichromatium gracile
49030
-
2 * 49030, nanospray ionization mass spectrometry
Marichromatium gracile
98000
-
gel filtration
Marichromatium gracile
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glutathione amide disulfide + NADH + H+
Marichromatium gracile
-
2 glutathione amide + NAD+
-
-
?
glutathione amide disulfide + NADH + H+
Marichromatium gracile DSM 1712
-
2 glutathione amide + NAD+
-
-
?
glutathione amide disulfide + NADPH + H+
Marichromatium gracile
-
2 glutathione amide + NADP+
-
-
?
glutathione amide disulfide + NADPH + H+
Marichromatium gracile DSM 1712
-
2 glutathione amide + NADP+
-
-
?
glutathione disulfide + NADH + H+
Marichromatium gracile
-
2 glutathione + NAD+
-
-
?
glutathione disulfide + NADH + H+
Marichromatium gracile DSM 1712
-
2 glutathione + NAD+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Marichromatium gracile
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glutathione amide disulfide + NADH + H+
-
698716
Marichromatium gracile
2 glutathione amide + NAD+
-
-
-
?
glutathione amide disulfide + NADH + H+
-
698716
Marichromatium gracile DSM 1712
2 glutathione amide + NAD+
-
-
-
?
glutathione amide disulfide + NADPH + H+
-
698716
Marichromatium gracile
2 glutathione amide + NADP+
-
-
-
?
glutathione amide disulfide + NADPH + H+
-
698716
Marichromatium gracile DSM 1712
2 glutathione amide + NADP+
-
-
-
?
glutathione disulfide + NADH + H+
-
698716
Marichromatium gracile
2 glutathione + NAD+
-
-
-
?
glutathione disulfide + NADH + H+
-
698716
Marichromatium gracile DSM 1712
2 glutathione + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 49028, calculation from sequence; 2 * 49030, nanospray ionization mass spectrometry
Marichromatium gracile
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.14
-
NADPH
pH 7.1, 25°C
Marichromatium gracile
146
-
glutathione disulfide
pH 7.1, 25°C
Marichromatium gracile
186.8
-
NADH
pH 7.1, 25°C
Marichromatium gracile
249.7
-
glutathione amide disulfide
pH 7.1, 25°C
Marichromatium gracile
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.07
-
NADPH
pH 7.1, 25°C
Marichromatium gracile
21.2
-
glutathione disulfide
pH 7.1, 25°C
Marichromatium gracile
2574
-
glutathione amide disulfide
pH 7.1, 25°C
Marichromatium gracile
14150
-
NADH
pH 7.1, 25°C
Marichromatium gracile
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.07
-
NADPH
pH 7.1, 25°C
Marichromatium gracile
21.2
-
glutathione disulfide
pH 7.1, 25°C
Marichromatium gracile
2574
-
glutathione amide disulfide
pH 7.1, 25°C
Marichromatium gracile
14150
-
NADH
pH 7.1, 25°C
Marichromatium gracile
Other publictions for EC 1.8.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
699521
van Petegem
Understanding nicotinamide din ...
Marichromatium gracile
J. Mol. Biol.
374
883-999
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695313
Vergauwen
Crystallization and preliminar ...
Marichromatium gracile
Acta Crystallogr. Sect. D
58
339-340
2002
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698716
Vergauwen
Characterization of glutathion ...
Marichromatium gracile
J. Biol. Chem.
276
20890-20897
2001
-
-
1
-
-
-
-
4
-
-
3
6
-
5
-
-
1
-
-
-
-
-
6
1
-
-
-
4
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
-
-
3
6
-
-
-
1
-
-
-
-
6
1
-
-
-
4
-
-
-
-
-
-
-
-
4
4