Literature summary for 1.7.7.1 extracted from

Terauchi, A.M.; Lu, S.-F.; Zaffagnini, M.; Tappa, S.; Hirasawa, M.; Tripathy, J.N.; Knaff, D.B.; Farmer, P.J.; Lemaire, S.D.; Hase, T.; Merchant, S.S.
Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii (2009), J. Biol. Chem., 284, 25867-25878 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0173	-	nitrite	cosubstrate Fdx2, pH 7.5, temperature not specified in the publication	Synechocystis sp. PCC 6803
0.0255	-	nitrite	cosubstrate Fdx2, pH 7.5, temperature not specified in the publication	Chlamydomonas reinhardtii
0.029	-	nitrite	cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication	Chlamydomonas reinhardtii
0.0415	-	nitrite	cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication	Synechocystis sp. PCC 6803

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	-	-	-
Synechocystis sp. PCC 6803	Q55366	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrite + 6 reduced Fdx2	Fdx2 i.e. Chlamydomonas reinhardtii plant-type isoform ferredoxin 2	Synechocystis sp. PCC 6803	ammonia + 2 H2O + 6 oxidized Fdx2	-	?
nitrite + 6 reduced Fdx2	Fdx2 i.e. Chlamydomonas reinhardtii plant-type isoform ferredoxin 2. Fdx2 is a more specific electron donor to NiR than ferredoxin	Chlamydomonas reinhardtii	ammonia + 2 H2O + 6 oxidized Fdx2	-	?
nitrite + 6 reduced ferredoxin	-	Chlamydomonas reinhardtii	ammonia + 2 H2O + 6 oxidized ferredoxin	-	?
nitrite + 6 reduced ferredoxin	-	Synechocystis sp. PCC 6803	ammonia + 2 H2O + 6 oxidized ferredoxin	-	?

Synonyms

Synonyms	Comment	Organism
NiR	-	Chlamydomonas reinhardtii
NirA	-	Synechocystis sp. PCC 6803

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.35	-	nitrite	cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication	Chlamydomonas reinhardtii
14.6	-	nitrite	cosubstrate Fdx2, pH 7.5, temperature not specified in the publication	Chlamydomonas reinhardtii
480	-	nitrite	cosubstrate Fdx2, pH 7.5, temperature not specified in the publication	Synechocystis sp. PCC 6803
666	-	nitrite	cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication	Synechocystis sp. PCC 6803

General Information

General Information	Comment	Organism
physiological function	the abundance of NiR polypeptide is increased at least 15fold in nitrate-grown cells	Chlamydomonas reinhardtii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
47	-	nitrite	cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication	Chlamydomonas reinhardtii
573	-	nitrite	cosubstrate Fdx2, pH 7.5, temperature not specified in the publication	Chlamydomonas reinhardtii
13900	-	nitrite	cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication	Synechocystis sp. PCC 6803
27700	-	nitrite	cosubstrate Fdx2, pH 7.5, temperature not specified in the publication	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)