BRENDA - Enzyme Database show
show all sequences of 1.6.99.3

Purification and characterization of NADH dehydrogenase from Bacillus subtilis

Bergsma, J.; van Dongen, M.B.M.; Konings, W.N.; Eur. J. Biochem. 128, 151-157 (1982)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
adenosine
slight inhibition
Bacillus subtilis
deoxyadenosine
slight inhibition
Bacillus subtilis
nicotinic acid amide
slight inhibition
Bacillus subtilis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Bacillus subtilis
16020
-
vesicular fraction
-
Bacillus subtilis
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
x * 64000, SDS-PAGE
Bacillus subtilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
W23
-
Bacillus subtilis W23
-
W23
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
24.67
-
-
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
-
392820
Bacillus subtilis
NAD+ + ?
-
-
-
?
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
-
392820
Bacillus subtilis W23
NAD+ + ?
-
-
-
?
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
less than 6% of the activity with NADH
392820
Bacillus subtilis
NADP+ + ?
-
-
-
?
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
less than 6% of the activity with NADH
392820
Bacillus subtilis W23
NADP+ + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 64000, SDS-PAGE
Bacillus subtilis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains 1 molecule FAD per molecule
Bacillus subtilis
NADH
-
Bacillus subtilis
NADPH
less than 6% of the activity with NADH in reaction with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains 1 molecule FAD per molecule
Bacillus subtilis
NADH
-
Bacillus subtilis
NADPH
less than 6% of the activity with NADH in reaction with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
adenosine
slight inhibition
Bacillus subtilis
deoxyadenosine
slight inhibition
Bacillus subtilis
nicotinic acid amide
slight inhibition
Bacillus subtilis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Bacillus subtilis
16020
-
vesicular fraction
-
Bacillus subtilis
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
x * 64000, SDS-PAGE
Bacillus subtilis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
24.67
-
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
-
392820
Bacillus subtilis
NAD+ + ?
-
-
-
?
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
-
392820
Bacillus subtilis W23
NAD+ + ?
-
-
-
?
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
less than 6% of the activity with NADH
392820
Bacillus subtilis
NADP+ + ?
-
-
-
?
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
less than 6% of the activity with NADH
392820
Bacillus subtilis W23
NADP+ + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 64000, SDS-PAGE
Bacillus subtilis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
Bacillus subtilis
Other publictions for EC 1.6.99.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741964
Villegas
FAD binding properties of a c ...
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Biochim. Biophys. Acta
1844
576-584
2014
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724488
Villegas
Maintenance and thermal stabil ...
Arabidopsis thaliana, Marchantia polymorpha, Nicotiana tabacum, Synechocystis sp., Zea mays
Biochimie
95
382-387
2013
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14
14
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746936
Lakhal
Oxygen uptake rates in the hy ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Arch. Microbiol.
193
429-438
2011
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701758
Do
Engineering Escherichia coli f ...
Trichomonas vaginalis
Appl. Biochem. Biotechnol.
153
21-33
2009
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-
1
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-
6
-
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3
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7
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1
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4
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4
1
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3
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1
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6
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3
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1
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4
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4
1
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3
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685693
Chakraborty
Two proteins with diaphorase a ...
Moorella thermoacetica
Biosci. Biotechnol. Biochem.
72
877-879
2008
-
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1
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2
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1
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1
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1
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692853
Platt
Proteomic, microarray, and sig ...
Pseudomonas aeruginosa
J. Bacteriol.
190
2739-2758
2008
-
-
1
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1
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1
-
4
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677088
Kitazume
Involvement of Lys-308 in the ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) YN-1
Res. Microbiol.
157
956-959
2006
-
-
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2
-
1
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3
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658494
Shiraki
Involvement of glycine and asp ...
Bacillus sp. YN-1
Curr. Microbiol.
46
432-434
2003
-
-
1
-
5
-
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2
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2
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1
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5
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2
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392824
Marques
Activation of a NADH dehydroge ...
Homo sapiens
Biol. Signals
6
52-61
1997
1
-
-
-
-
-
-
-
1
-
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1
-
2
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1
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2
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2
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392825
Thiagalingam
-
Purification and characterizat ...
Bacillus megaterium
Can. J. Microbiol.
39
826-833
1993
-
-
-
-
-
-
10
4
1
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1
1
-
1
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1
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1
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5
1
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1
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2
1
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2
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10
1
4
1
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1
-
5
1
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1
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392819
Nisimoto
NADH dehydrogenase from bovine ...
Bos taurus
J. Biol. Chem.
261
285-290
1986
-
-
-
-
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3
2
2
-
2
-
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3
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1
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1
1
1
4
1
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3
1
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3
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1
2
2
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2
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1
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1
1
1
4
1
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392812
Borneleit
-
Purification and properties of ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
722
94-101
1983
1
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392820
Bergsma
Purification and characterizat ...
Bacillus subtilis, Bacillus subtilis W23
Eur. J. Biochem.
128
151-157
1982
-
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3
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4
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1
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8
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1
1
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1
1
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3
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1
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1
1
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1
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392811
Kitajima
Purification and properties of ...
Homo sapiens
Arch. Biochem. Biophys.
210
330-339
1981
-
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-
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7
2
1
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2
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1
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1
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1
1
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6
1
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1
2
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1
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3
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7
1
2
1
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2
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1
1
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6
1
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1
2
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392810
Wang
Human erythrocyte NADH: (accep ...
Homo sapiens
Biochim. Biophys. Acta
616
22-29
1980
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3
2
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392805
Adachi
Study on the reduced pyridine ...
Bos taurus
Biochim. Biophys. Acta
268
629-637
1972
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1
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392821
Ramanarayanan
Purification & properties of a ...
Agrobacterium tumefaciens
Indian J. Biochem. Biophys.
8
214-218
1971
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1
1
1
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4
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1
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1
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392807
Kaniuga
The Transformation of mitochon ...
Sus scrofa
Biochim. Biophys. Acta
73
550-564
1963
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2
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1
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3
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1
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2
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1
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3
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1
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