Protein Variants | Comment | Organism |
---|---|---|
additional information | NDH2 gene silencing using tetracycline-inducible RNAi | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone | Trypanosoma brucei | - |
NAD+ + ubiquinol | - |
? | |
NADH + H+ + ubiquinone | Trypanosoma brucei 29-13 | - |
NAD+ + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Trypanosoma brucei 29-13 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone | - |
Trypanosoma brucei | NAD+ + ubiquinol | - |
? | |
NADH + H+ + ubiquinone | - |
Trypanosoma brucei 29-13 | NAD+ + ubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
alternative NADH dehydrogenase | - |
Trypanosoma brucei |
alternative rotenone-insensitive NADH:Q oxidoreductase | - |
Trypanosoma brucei |
NADH: ubiquinone-2 oxidoreductase | - |
Trypanosoma brucei |
NADHQ2 | - |
Trypanosoma brucei |
NDH2 | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
malfunction | the induced RNAi cell line exhibits slower growth, decreased mitochondrial membrane potential and lower sensitivity of respiration to inhibitors. Mitochondrial membrane potential is altered in NDH2 knockdown. Growth phenotype of procyclic Trypanosoma brucei interfered against NDH2, overview | Trypanosoma brucei |
physiological function | NDH2 is a part of the respiratory chain that does not pump protons across the inner membrane. NDH2 is the major NADH: ubiquinone oxidoreductase responsible for cytosolic and not for mitochondrial NAD+ regeneration in the mitochondrion of procyclic Trypanosoma brucei. thatNDH2is facing intermembrane space, rendering complex I responsible only for the regeneration of matrix NAD+. Ability of NDH2 to fully compensate for the loss of complex I. NDH2 is essential for the maintenance of mitochondrial membrane potential in procyclic Trypanosoma brucei | Trypanosoma brucei |