Application | Comment | Organism |
---|---|---|
degradation | TcpB is acting as a quinone reductase for 6-chlorohydroxyquinone reduction during 2,4,6-trichlorophenol degradation, a toxic pollutant | Cupriavidus pinatubonensis JMP134 |
Cloned (Comment) | Organism |
---|---|
into the pET30-LIC vector, overexpressed in Escherichia coli BL21(DE3) | Cupriavidus pinatubonensis JMP134 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
FMN | - |
Cupriavidus pinatubonensis JMP134 | |
0.0863 | - |
menadione | - |
Cupriavidus pinatubonensis JMP134 | |
0.2311 | - |
NADH | - |
Cupriavidus pinatubonensis JMP134 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
2 * 21000, SDS-PAGE | Cupriavidus pinatubonensis JMP134 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus pinatubonensis JMP134 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified as a C-terminal His tag fusion by ultracentrifugation and gel filtration, to apparent homogeneity | Cupriavidus pinatubonensis JMP134 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
with FMN as substrate | Cupriavidus pinatubonensis JMP134 |
8.1 | - |
with ferricyanide and FAD as substrates | Cupriavidus pinatubonensis JMP134 |
35.3 | - |
with menadione and FAD as substrates | Cupriavidus pinatubonensis JMP134 |
179.1 | - |
with ferricyanide and FMN as substrates | Cupriavidus pinatubonensis JMP134 |
911.4 | - |
with menadione and FMN as substrates | Cupriavidus pinatubonensis JMP134 |
Storage Stability | Organism |
---|---|
-80°C, KPi buffer, 10% glycerol, several months | Cupriavidus pinatubonensis JMP134 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-6-hydroxy-1,4-benzoquinone + NADH | - |
Cupriavidus pinatubonensis JMP134 | 6-chlorobenzene-1,2,4-triol + NAD+ | - |
? | |
FAD + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
ferricyanide + FAD + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
ferricyanide + FMN + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
FMN + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
menadione + FAD + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
menadione + FMN + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 21000, SDS-PAGE | Cupriavidus pinatubonensis JMP134 |
Synonyms | Comment | Organism |
---|---|---|
Quinone reductase | - |
Cupriavidus pinatubonensis JMP134 |
TcpB | - |
Cupriavidus pinatubonensis JMP134 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
350.7 | - |
menadione | - |
Cupriavidus pinatubonensis JMP134 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Cupriavidus pinatubonensis JMP134 | |
FMN | - |
Cupriavidus pinatubonensis JMP134 | |
NADH | - |
Cupriavidus pinatubonensis JMP134 |