Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3'-methylene-bis(4-hydroxycoumarin) | 0.04 mM, complete inhibition | Glycine max | |
Alizarin Red S | 0.04 mM, 38% inhibition | Glycine max | |
Cibacron Blue F3G-A | 0.04 mM, 75% inhibition | Glycine max | |
Procion blue MX-R | 0.04 mM, 75% inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
NADPH | - |
Glycine max | |
0.341 | - |
NADH | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21900 | - |
2 * 21900, SDS-PAGE | Glycine max |
61600 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Agarose cibacron blue, AH-Sepharose, CM-cellulose, Sephacryl S-200 HR | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2469 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,8-dihydroxynaphthoquinone + NAD(P)H | - |
Glycine max | 5,8-dihydroxynaphthohydroquinone + NAD(P)+ | - |
? | |
6-hydroxydopaminequinone + NAD(P)H | - |
Glycine max | 6-hydroxydopaminehydroquinone + NAD(P)+ | - |
? | |
6-hydroxydopaquinone + NAD(P)H | - |
Glycine max | 6-hydroxydopahydroquinone + NAD(P)+ | - |
? | |
juglone + NAD(P)H | - |
Glycine max | dihydrojuglone + NAD(P)+ | - |
? | |
K3[Fe(CN)6] + NAD(P)H | - |
Glycine max | K4[Fe(CN)6] + NAD(P)+ | - |
? | |
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Glycine max | NAD(P)+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
NADH + H+ + 2,6-dichlorophenolindophenol | - |
Glycine max | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 21900, SDS-PAGE | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 43 | decrease above | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
60% activity at pH 9.0 | Glycine max |