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Literature summary for 1.6.1.2 extracted from

  • Fristedt, U.; Rydström, J.; Persson, B.
    Evidence for a nicotineamide nucleotide transhydrogenase in Klebsiella pneumoniae (1994), Biochem. Biophys. Res. Commun., 198, 928-932.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
palmitoyl-CoA
-
Escherichia coli
palmitoyl-CoA competitive vs. NADPH Klebsiella pneumoniae
Phenylarsine oxide 0.48 mM, 50% inhibition of acetylpyridine adenine dinucleotide reduction in inside-out membrane vesicles after 5 min Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ not clear whether this reflects a general salt effect or a Li+ specific effect Klebsiella pneumoniae
Na+ not clear whether this reflects a general salt effect or a Na+ specific effect Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000, immunoblot with antibodies against beef heart enzyme Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Klebsiella pneumoniae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.26
-
activity in inside-out membrane vesicles at pH 7.4, presence of an uncoupler i.e. carbonylcyanide-m-chlorophylhydrazone results in 2fold stimulation Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + NADP+
-
Escherichia coli NAD+ + NADPH
-
?
NADH + NADP+
-
Klebsiella pneumoniae NAD+ + NADPH
-
r
NADPH + oxidized 3-acetylpyridine adenine dinucleotide
-
Escherichia coli NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
r
NADPH + oxidized 3-acetylpyridine adenine dinucleotide
-
Klebsiella pneumoniae NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
r

Subunits

Subunits Comment Organism
? x * 54000, immunoblot with antibodies against beef heart enzyme Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 reduction of oxidized acetylpyridine adenine dinucleotide by NADPH in inside-out membrane vesicles Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NAD+
-
Klebsiella pneumoniae
NADH
-
Escherichia coli
NADH
-
Klebsiella pneumoniae
NADP+
-
Escherichia coli
NADP+
-
Klebsiella pneumoniae
NADPH
-
Escherichia coli
NADPH
-
Klebsiella pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00125
-
palmitoyl-CoA
-
Klebsiella pneumoniae
0.007
-
palmitoyl-CoA
-
Escherichia coli