Literature summary for 1.5.99.B2 extracted from

Kawakami, R.; Noguchi, C.; Higashi, M.; Sakuraba, H.; Ohshima, T.
Comparative analysis of the catalytic components in the archaeal dye-linked L-proline dehydrogenase complexes (2013), Appl. Microbiol. Biotechnol., 97, 3419-3427.

Search for more literature for 1.5.99.B2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells	Pyrococcus furiosus
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells	Pyrococcus horikoshii
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells	Thermococcus kodakarensis
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells	Thermococcus profundus

Inhibitors

Inhibitors	Comment	Organism
L-pyroglutamate	-	Pyrococcus furiosus
L-pyroglutamate	-	Pyrococcus horikoshii
L-pyroglutamate	-	Thermococcus kodakarensis
L-pyroglutamate	-	Thermococcus profundus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.174	-	L-proline	PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus furiosus
0.334	-	L-proline	PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus horikoshii
0.689	-	L-proline	PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus furiosus
1.46	-	L-proline	TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus kodakarensis
2.38	-	L-proline	TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus kodakarensis
2.46	-	L-proline	TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus profundus
2.5	5	L-proline	PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus horikoshii
3.5	-	L-proline	TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus profundus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	-	-	-
Pyrococcus horikoshii	-	-	-
Pyrococcus horikoshii OT-3	-	-	-
Thermococcus kodakarensis	-	-	-
Thermococcus kodakarensis KOD1 JCM 12380	-	-	-
Thermococcus profundus	Q76M73	-	-
Thermococcus profundus DSM 9503	Q76M73	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-chelating column chromatography	Pyrococcus furiosus
Ni-chelating column chromatography	Pyrococcus horikoshii
Ni-chelating column chromatography	Thermococcus kodakarensis
Ni-chelating column chromatography	Thermococcus profundus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-proline + 2,6-dichlorophenolindophenol + H2O	the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor	Pyrococcus furiosus	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
L-proline + 2,6-dichlorophenolindophenol + H2O	the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor	Pyrococcus horikoshii	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
L-proline + 2,6-dichlorophenolindophenol + H2O	the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor	Thermococcus kodakarensis	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
L-proline + 2,6-dichlorophenolindophenol + H2O	the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor	Thermococcus profundus	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
L-proline + 2,6-dichlorophenolindophenol + H2O	the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor	Thermococcus profundus DSM 9503	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
L-proline + 2,6-dichlorophenolindophenol + H2O	the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor	Pyrococcus horikoshii OT-3	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
L-proline + 2,6-dichlorophenolindophenol + H2O	the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor	Thermococcus kodakarensis KOD1 JCM 12380	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?
L-proline + 4-iodonitrotetrazolium violet + H2O	-	Pyrococcus furiosus	DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet	-	?
L-proline + 4-iodonitrotetrazolium violet + H2O	-	Pyrococcus horikoshii	DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet	-	?
L-proline + 4-iodonitrotetrazolium violet + H2O	-	Thermococcus kodakarensis	DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet	-	?
L-proline + 4-iodonitrotetrazolium violet + H2O	-	Thermococcus profundus	DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet	-	?
L-proline + 4-iodonitrotetrazolium violet + H2O	-	Thermococcus profundus DSM 9503	DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet	-	?
L-proline + 4-iodonitrotetrazolium violet + H2O	-	Pyrococcus horikoshii OT-3	DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet	-	?
L-proline + 4-iodonitrotetrazolium violet + H2O	-	Thermococcus kodakarensis KOD1 JCM 12380	DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet	-	?
L-proline + ferricyanide	the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor	Pyrococcus furiosus	DELTA1-pyrroline-5-carboxylate + ferrocyanide	-	?
L-proline + ferricyanide	the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor	Pyrococcus horikoshii	DELTA1-pyrroline-5-carboxylate + ferrocyanide	-	?
L-proline + ferricyanide	the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor	Thermococcus kodakarensis	DELTA1-pyrroline-5-carboxylate + ferrocyanide	-	?
L-proline + ferricyanide	the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor	Thermococcus profundus	DELTA1-pyrroline-5-carboxylate + ferrocyanide	-	?
L-proline + ferricyanide	the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor	Thermococcus profundus DSM 9503	DELTA1-pyrroline-5-carboxylate + ferrocyanide	-	?
L-proline + ferricyanide	the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor	Pyrococcus horikoshii OT-3	DELTA1-pyrroline-5-carboxylate + ferrocyanide	-	?
L-proline + ferricyanide	the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor	Thermococcus kodakarensis KOD1 JCM 12380	DELTA1-pyrroline-5-carboxylate + ferrocyanide	-	?
additional information	D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates	Pyrococcus furiosus	?	-	?
additional information	D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates	Pyrococcus horikoshii	?	-	?
additional information	D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates	Thermococcus kodakarensis	?	-	?
additional information	D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates	Thermococcus profundus	?	-	?
additional information	D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates	Thermococcus profundus DSM 9503	?	-	?
additional information	D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates	Pyrococcus horikoshii OT-3	?	-	?
additional information	D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates	Thermococcus kodakarensis KOD1 JCM 12380	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 44000-45000, SDS-PAGE	Pyrococcus furiosus
?	x * 44000-45000, SDS-PAGE	Pyrococcus horikoshii
?	x * 44000-45000, SDS-PAGE	Thermococcus kodakarensis
?	x * 44000-45000, SDS-PAGE	Thermococcus profundus

Synonyms

Synonyms	Comment	Organism
L-proline dehydrogenase	-	Pyrococcus furiosus
L-proline dehydrogenase	-	Pyrococcus horikoshii
L-proline dehydrogenase	-	Thermococcus kodakarensis
L-proline dehydrogenase	-	Thermococcus profundus
PDH	-	Pyrococcus furiosus
PDH	-	Pyrococcus horikoshii
PDH	-	Thermococcus kodakarensis
PDH	-	Thermococcus profundus
PF1246	alpha4beta4-type beta subunit of L-proline dehydrogenase	Pyrococcus furiosus
PF1798	alphabetagammadelta-type beta subunit of L-proline dehydrogenase	Pyrococcus furiosus
PH1364	alpha4beta4-type beta subunit of L-proline dehydrogenase	Pyrococcus horikoshii
PH1751	alphabetagammadelta-type beta subunit of L-proline dehydrogenase	Pyrococcus horikoshii
PRODH	-	Pyrococcus furiosus
PRODH	-	Pyrococcus horikoshii
PRODH	-	Thermococcus kodakarensis
PRODH	-	Thermococcus profundus
TK0117	alpha4beta4-type beta subunit of L-proline dehydrogenase	Thermococcus kodakarensis
TK0122	alphabetagammadelta-type beta subunit of L-proline dehydrogenase	Thermococcus kodakarensis
TPpdhB	alphabetagammadelta-type beta subunit of L-proline dehydrogenase	Thermococcus profundus
TPpdhB2	alpha4beta4-type beta subunit of L-proline dehydrogenase	Thermococcus profundus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	alphabetagammadelta-type beta subunit of L-proline dehydrogenase	Pyrococcus furiosus
65	-	alphabetagammadelta-type beta subunit of L-proline dehydrogenase	Pyrococcus horikoshii
65	-	alphabetagammadelta-type beta subunit of L-proline dehydrogenase	Thermococcus kodakarensis
65	-	alphabetagammadelta-type beta subunit of L-proline dehydrogenase	Thermococcus profundus
80	-	alpha4beta4-type beta subunit of L-proline dehydrogenase	Pyrococcus furiosus
80	-	alpha4beta4-type beta subunit of L-proline dehydrogenase	Pyrococcus horikoshii
80	-	alpha4beta4-type beta subunit of L-proline dehydrogenase	Thermococcus kodakarensis
80	-	alpha4beta4-type beta subunit of L-proline dehydrogenase	Thermococcus profundus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase nearly loses all of its activity under the same conditions	Pyrococcus horikoshii
70	-	the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase retains 100% activity under the same conditions	Pyrococcus furiosus
70	-	the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase shows 68% activity under the same conditions	Thermococcus profundus
70	-	the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase shows 76% activity under the same conditions	Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
1.96	-	L-proline	PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus horikoshii
3.49	-	L-proline	PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus furiosus
3.91	-	L-proline	PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus furiosus
6.22	-	L-proline	TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus profundus
11	-	L-proline	PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus horikoshii
11.6	-	L-proline	TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus kodakarensis
27.6	-	L-proline	TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus kodakarensis
27.9	-	L-proline	TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus profundus

Cofactor

Cofactor	Comment	Organism
FAD	holoprotein contains one FAD per molecule	Pyrococcus furiosus
FAD	holoprotein contains one FAD per molecule	Pyrococcus horikoshii
FAD	holoprotein contains one FAD per molecule	Thermococcus kodakarensis
FAD	holoprotein contains one FAD per molecule	Thermococcus profundus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
24	-	L-pyroglutamate	PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus horikoshii
49.4	-	L-pyroglutamate	TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus kodakarensis
55.5	-	L-pyroglutamate	PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus horikoshii
56.1	-	L-pyroglutamate	TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus profundus
68.3	-	L-pyroglutamate	PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus furiosus
122	-	L-pyroglutamate	TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus profundus
129	-	L-pyroglutamate	PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Pyrococcus furiosus
167	-	L-pyroglutamate	TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C	Thermococcus kodakarensis