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Literature summary for 1.5.99.B2 extracted from
- Purification and properties of the bifunctional proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase from Pseudomonas aeruginosa (1982), Eur. J. Biochem., 129, 67-75.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Pseudomonas aeruginosa |  |
| Hg2+ | - |
Pseudomonas aeruginosa | |
| Mg2+ | slightly | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Zn2+ | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
FAD | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 119000 | - |
2 * 119000, SDS-PAGE | Pseudomonas aeruginosa |
| 242000 | - |
gel filtration, native PAGE leads to two active forms of 24000 and 47000 | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-proline + acceptor + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
(S)-1-pyrroline-5-carboxylate + reduced acceptor | - |
? | |
| L-proline + acceptor + H2O | Pseudomonas aeruginosa | - |
(S)-1-pyrroline-5-carboxylate + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol | also active as 1-pyrroline-5-carboxylate dehydrogenase | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.75 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.26 | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.6, 50% glycerol, 1 month | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-proline + acceptor + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | (S)-1-pyrroline-5-carboxylate + reduced acceptor | - |
? | |
| L-proline + acceptor + H2O | - |
Pseudomonas aeruginosa | (S)-1-pyrroline-5-carboxylate + reduced acceptor | - |
? | |
| L-proline + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c | Salmonella enterica subsp. enterica serovar Typhimurium | (S)-1-pyrroline-5-carboxylate + reduced acceptor | - |
? | |
| L-proline + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c | Pseudomonas aeruginosa | (S)-1-pyrroline-5-carboxylate + reduced acceptor | - |
? | |
| L-proline + acceptor + H2O | highly specific for L-form of proline | Pseudomonas aeruginosa | (S)-1-pyrroline-5-carboxylate + reduced acceptor | - |
? | |
| L-proline + FAD | - |
Pseudomonas aeruginosa | (S)-1-pyrroline-5-carboxylate + FADH2 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 119000, SDS-PAGE | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
L-proline + phenazine methosulfate | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas aeruginosa | |
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