BRENDA - Enzyme Database
show all sequences of 1.5.8.2

Trimethylamine dehydrogenase from a methylotrophic bacterium. I. Isolation and steady-state kinetics

Steenkamp, D.J.; Mallinson, J.; Biochim. Biophys. Acta 429, 7005-719 (1976)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetaldehyde
noncompetitive inhibition with respect to diethylamine, uncompetitive inhibition with respect to phenazine methosulfate
Methylophilus methylotrophus
Diethylamine
noncompetitive product inhibition with respect to phenazine methosulfate
Methylophilus methylotrophus
ethylamine
noncompetitive product inhibition with respect to phenazine methosulfate
Methylophilus methylotrophus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
146800
-
sedimentation equilibrium ultracentrifugation
Methylophilus methylotrophus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
trimethylamine + H2O + electron acceptor
Methylophilus methylotrophus
ping-ping reaction mechanism
dimethylamine + formaldehyde + reduced electron acceptor
-
Methylophilus methylotrophus
?
trimethylamine + H2O + electron acceptor
Methylophilus methylotrophus W3A1
ping-ping reaction mechanism
dimethylamine + formaldehyde + reduced electron acceptor
-
Methylophilus methylotrophus W3A1
?
Organism
Organism
UniProt
Commentary
Textmining
Methylophilus methylotrophus
-
-
-
Methylophilus methylotrophus W3A1
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
to homogeneity, chromatography steps
Methylophilus methylotrophus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Methylophilus methylotrophus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.07
-
-
Methylophilus methylotrophus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
diethylamine + H2O + phenazine methosulfate
-
393920
Methylophilus methylotrophus
ethylamine + acetaldehyde + reduced phenazine methosulfate
-
393920
Methylophilus methylotrophus
?
diethylamine + H2O + phenazine methosulfate
-
393920
Methylophilus methylotrophus W3A1
ethylamine + acetaldehyde + reduced phenazine methosulfate
-
393920
Methylophilus methylotrophus W3A1
?
trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
393920
Methylophilus methylotrophus
dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
393920
Methylophilus methylotrophus W3A1
dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
trimethylamine + H2O + electron acceptor
ping-ping reaction mechanism
393920
Methylophilus methylotrophus
dimethylamine + formaldehyde + reduced electron acceptor
-
393920
Methylophilus methylotrophus
?
trimethylamine + H2O + electron acceptor
ping-ping reaction mechanism
393920
Methylophilus methylotrophus W3A1
dimethylamine + formaldehyde + reduced electron acceptor
-
393920
Methylophilus methylotrophus W3A1
?
trimethylamine + H2O + phenazine methosulfate
-
393920
Methylophilus methylotrophus
dimethylamine + formaldehyde + reduced phenazine methosulfate
-
393920
Methylophilus methylotrophus
?
trimethylamine + H2O + phenazine methosulfate
-
393920
Methylophilus methylotrophus W3A1
dimethylamine + formaldehyde + reduced phenazine methosulfate
-
393920
Methylophilus methylotrophus W3A1
?
Subunits
Subunits
Commentary
Organism
dimer
non-identical subunits of 70000-80000, SDS-PAGE
Methylophilus methylotrophus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetaldehyde
noncompetitive inhibition with respect to diethylamine, uncompetitive inhibition with respect to phenazine methosulfate
Methylophilus methylotrophus
Diethylamine
noncompetitive product inhibition with respect to phenazine methosulfate
Methylophilus methylotrophus
ethylamine
noncompetitive product inhibition with respect to phenazine methosulfate
Methylophilus methylotrophus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
146800
-
sedimentation equilibrium ultracentrifugation
Methylophilus methylotrophus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
trimethylamine + H2O + electron acceptor
Methylophilus methylotrophus
ping-ping reaction mechanism
dimethylamine + formaldehyde + reduced electron acceptor
-
Methylophilus methylotrophus
?
trimethylamine + H2O + electron acceptor
Methylophilus methylotrophus W3A1
ping-ping reaction mechanism
dimethylamine + formaldehyde + reduced electron acceptor
-
Methylophilus methylotrophus W3A1
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography steps
Methylophilus methylotrophus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Methylophilus methylotrophus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.07
-
-
Methylophilus methylotrophus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
diethylamine + H2O + phenazine methosulfate
-
393920
Methylophilus methylotrophus
ethylamine + acetaldehyde + reduced phenazine methosulfate
-
393920
Methylophilus methylotrophus
?
diethylamine + H2O + phenazine methosulfate
-
393920
Methylophilus methylotrophus W3A1
ethylamine + acetaldehyde + reduced phenazine methosulfate
-
393920
Methylophilus methylotrophus W3A1
?
trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
393920
Methylophilus methylotrophus
dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
393920
Methylophilus methylotrophus W3A1
dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
trimethylamine + H2O + electron acceptor
ping-ping reaction mechanism
393920
Methylophilus methylotrophus
dimethylamine + formaldehyde + reduced electron acceptor
-
393920
Methylophilus methylotrophus
?
trimethylamine + H2O + electron acceptor
ping-ping reaction mechanism
393920
Methylophilus methylotrophus W3A1
dimethylamine + formaldehyde + reduced electron acceptor
-
393920
Methylophilus methylotrophus W3A1
?
trimethylamine + H2O + phenazine methosulfate
-
393920
Methylophilus methylotrophus
dimethylamine + formaldehyde + reduced phenazine methosulfate
-
393920
Methylophilus methylotrophus
?
trimethylamine + H2O + phenazine methosulfate
-
393920
Methylophilus methylotrophus W3A1
dimethylamine + formaldehyde + reduced phenazine methosulfate
-
393920
Methylophilus methylotrophus W3A1
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
non-identical subunits of 70000-80000, SDS-PAGE
Methylophilus methylotrophus
Other publictions for EC 1.5.8.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711031
Liffourrena
Pseudomonas putida A ATCC 1263 ...
Pseudomonas putida, Pseudomonas putida ATCC 12633
Arch. Microbiol.
192
471-476
2010
1
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1
2
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2
-
13
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2
1
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4
-
1
1
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1
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1
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1
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2
1
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4
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1
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1
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-
-
-
-
-
-
-
685241
Burgess
Probing the dynamic interface ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochemistry
47
5168-5181
2008
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-
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-
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2
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44
-
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6
-
1
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2
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2
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2
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2
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6
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2
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-
656284
Shi
The interaction of trimethylam ...
Methylophilus methylotrophus
J. Biol. Chem.
280
20239-20246
2005
-
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1
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1
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1
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7
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1
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2
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1
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1
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1
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2
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1
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-
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-
-
-
-
-
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-
654192
Loechel
Using trimethylamine dehydroge ...
Methylophilus methylotrophus
Analyst
128
166-172
2003
-
1
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-
-
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3
1
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1
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1
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5
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1
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2
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1
1
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1
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1
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1
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1
1
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654971
Lu
A mechanism for substrate-indu ...
Methylophilus methylotrophus
Bioorg. Med. Chem. Lett.
13
4129-4132
2003
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1
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1
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1
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1
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4
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1
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3
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1
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655622
Kim
Isolation and characterization ...
Paracoccus sp.
FEMS Microbiol. Lett.
225
263-269
2003
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1
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5
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1
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3
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1
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1
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1
8
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3
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1
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393898
Jones
Electron transfer and conforma ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
277
8457-8465
2002
-
-
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3
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1
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2
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44
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1
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1
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1
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2
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393894
Kim
A novel denitrifying bacterial ...
Paracoccus sp., Paracoccus sp. T231
Arch. Microbiol.
176
271-277
2001
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7
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1
1
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1
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393896
Hille
Coupled electron/proton transf ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
276
31193-31201
2001
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44
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393897
Mitchell
Inactivation of C30A trimethyl ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochemistry
40
8523-8530
2001
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1
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1
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3
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1
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2
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47
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393895
Basran
Differential coupling through ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochemistry
39
9188-9200
2000
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1
1
2
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1
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393893
Basran
-
Reductive half-reaction of the ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochem. J.
341
307-314
1999
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393892
Ertrughrul
Probing the stabilizing role o ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Protein Eng.
11
447-455
1998
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1
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1
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393891
Wilson
An exposed tyrosine on the sur ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochemistry
36
41-48
1997
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1
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3
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4
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1
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44
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1
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393885
Falzon
Intramolecular electron transf ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochemistry
35
12111-12118
1996
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44
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393886
Huang
Reaction of the C30A mutant of ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
271
13401-13406
1996
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1
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1
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39
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393890
Cölfen
-
Homodimeric and expanded behav ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biophys. J.
24
159-164
1996
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39
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393889
Huang
The reaction of trimethylamine ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
270
23958-23965
1995
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393888
Scrutton
Assembly of redox centers in t ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
269
13942-13950
1994
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1
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3
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50
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2
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3
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-
393887
Barber
Correlation of X-ray deduced a ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
267
6611-6619
1992
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42
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4
-
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393899
Bellamy
Studies of crystalline trimeth ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
264
11887-11892
1989
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39
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393900
Barber
Microcoulometric analysis of t ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochem. J.
256
657-659
1988
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-
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1
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1
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47
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1
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2
-
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-
393901
Lim
Identification of ADP in the i ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
263
3075-3078
1988
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1
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1
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42
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2
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393902
Stevenson
Studies on the spin-spin inter ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochim. Biophys. Acta
869
81-88
1986
-
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42
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2
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-
393903
Lim
Three-dimensional structure of ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
261
15140-15146
1986
-
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1
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1
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2
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45
-
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1
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1
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2
1
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1
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1
1
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2
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1
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1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393904
Davidson
Electron transfer flavoprotein ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Bacteriol.
166
812-817
1986
-
-
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2
-
52
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1
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1
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2
-
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-
-
-
-
-
-
-
-
393925
Mathews
-
Crystal structure study of tri ...
Methylophilus methylotrophus
Flavins and Flavoproteins (Proc. Int. Symp. , 8th, Meeting Date 1984, 233-6, Bray, R. C. ; Engel, P. C. ; de Gruyter, M. S. G. eds. )
102
162926
1985
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1
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1
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1
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1
1
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-
393905
Lim
Molecular structure of trimeth ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
259
14458-14462
1984
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1
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1
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45
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2
-
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-
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-
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-
393879
Kasprzak
Identity of the subunits and t ...
Hyphomicrobium sp., Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochem. J.
211
535-541
1983
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1
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45
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2
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4
2
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1
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1
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4
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2
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4
2
-
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-
-
-
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-
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-
-
-
-
393884
Kasprzak
Localization of the major dehy ...
Hyphomicrobium sp., Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Bacteriol.
156
348-353
1983
-
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2
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4
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49
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2
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4
-
-
-
-
-
-
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-
-
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-
-
-
-
393908
Burton
-
The oxidation of methylated am ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
FEMS Microbiol. Lett.
17
185-190
1983
-
-
-
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1
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39
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2
-
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-
-
-
393878
Steenkamp
Mechanistic studies on the deh ...
Hyphomicrobium sp., Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochem. J.
207
241-252
1982
-
-
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2
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1
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4
-
50
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1
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2
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4
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3
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3
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4
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1
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2
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4
-
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-
-
-
-
393909
Steenkamp
Mechanistic studies on the deh ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochem. J.
207
233-239
1982
-
-
-
-
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1
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1
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2
-
48
-
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1
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1
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2
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1
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2
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2
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1
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1
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2
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1
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1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
393911
Lim
Crystallographic study of the ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Mol. Biol.
162
869-876
1982
-
-
-
1
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1
1
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-
42
-
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1
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2
1
-
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-
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1
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1
1
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1
1
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1
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393881
Steenkamp
Identification of the prosthet ...
Hyphomicrobium sp.
Biochem. Biophys. Res. Commun.
88
244-250
1979
-
-
-
-
-
-
1
-
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2
-
1
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1
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1
1
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2
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1
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1
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1
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2
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1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393913
Nagy
The reaction of phenylhydrazin ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
254
2684-2688
1979
-
-
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1
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2
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39
-
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2
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1
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2
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1
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393914
Kenney
Amino acid sequence of a cofac ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
FEBS Lett.
85
137-140
1978
-
-
-
-
-
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1
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2
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39
-
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1
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2
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1
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1
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2
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1
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2
-
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-
-
-
-
-
-
-
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-
-
-
-
393915
Steenkamp
The natural flavoprotein elect ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
253
4086-4089
1978
-
-
-
-
-
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-
-
-
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2
-
47
-
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1
-
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2
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-
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2
-
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2
-
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2
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393916
Steenkamp
Structure of the covalently bo ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
253
2818-2824
1978
-
-
-
-
-
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-
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-
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2
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39
-
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1
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2
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1
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1
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2
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393917
Steenkamp
A novel type of covalently bou ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
J. Biol. Chem.
253
2812-2817
1978
-
-
-
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-
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2
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39
-
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1
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2
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1
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1
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2
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393918
Steenkamp
Participation of the iron-sulp ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochem. J.
169
361-369
1978
-
-
-
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-
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1
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2
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39
-
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1
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2
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1
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1
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1
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2
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393919
Hill
Identification of the iron-sul ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Proc. Natl. Acad. Sci. USA
74
547-551
1977
-
-
-
-
-
-
-
-
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2
-
2
-
48
-
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1
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2
-
-
-
-
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1
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1
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-
-
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2
-
2
-
-
-
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393920
Steenkamp
-
Trimethylamine dehydrogenase f ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochim. Biophys. Acta
429
7005-719
1976
-
-
-
-
-
-
3
-
-
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1
2
-
39
-
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1
-
-
1
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
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1
2
-
-
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1
-
1
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393921
Colby
Enzymological aspects of the p ...
Methylophilus methylotrophus, no activity in Bacillus sp., Methylophilus methylotrophus W3A1, Methylophilus methylotrophus W6A
Biochem. J.
148
513-520
1975
-
-
-
-
-
-
-
-
-
-
-
3
-
43
-
-
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-
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1
2
-
3
-
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-
-
-
-
-
-
-
-
-
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-
-
-
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3
-
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1
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393922
Large
An enzymatic method for the mi ...
Hyphomicrobium vulgare, Hyphomicrobium vulgare NQ-521
Anal. Biochem.
64(1)
304-310
1975
-
1
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2
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1
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2
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-
393923
Colby
Purification and properties of ...
methylotrophic bacterium, methylotrophic bacterium 4B6
Biochem. J.
143
555-567
1974
-
-
-
-
-
-
27
9
-
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1
1
-
5
-
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1
-
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1
1
1
14
-
-
-
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1
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
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27
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9
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1
1
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1
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1
1
1
14
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1
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1
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393924
Colby
Trimethylamine metabolism in o ...
methylotrophic bacterium, no activity in methylotrophic bacterium, no activity in Pseudomonas sp., methylotrophic bacterium C2A1, no activity in Pseudomonas sp. 3A2, methylotrophic bacterium 4B6
Biochem. J.
132
101-112
1973
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7
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1
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3
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1
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3
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