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Literature summary for 1.5.3.26 extracted from

  • Saxena, A.; Saxena, P.; Monnier, V.
    Purification and characterization of a membrane-bound deglycating enzyme (1-deoxyfructosyl alkyl amino acid oxidase, EC 1.5.3) from a Pseudomonas sp. soil strain (1996), J. Biol. Chem., 271, 32803-32809 .
    View publication on PubMed
Search for more literature for 1.5.3.26

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ 10 mM, 60% of initial activity Pseudomonas sp.
azide complete inhibitiion Pseudomonas sp.
Cu2+ 10 mM, 60% of initial activity Pseudomonas sp.
Cyanate complete inhibition Pseudomonas sp.
dithiothreitol 10 mM, 87% of initial activity Pseudomonas sp.
Hg2+ 10 mM, complete loss of activity Pseudomonas sp.
additional information not inhibitory: EDTA, diethylenetriamine pentaacetic acid Pseudomonas sp.
Zn2+ 10 mM, 67% of initial activity Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 Nepsilon-(1-deoxyfructosyl)-aminocaproate pH 7.4, 25°C Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Pseudomonas sp. 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM, 17% of initial activity Pseudomonas sp.
Mn2+ 10 mM, 118% of initial activity Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme can be solubilized in active form using 10 mM 3-[(3-chlolamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate aided by 2 M NaCl and 10% glycerol (27% optimal solubilization yield) Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.38
-
 pH 7.4, 25°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nepsilon-(1-deoxyfructosyl)-aminocaproate + O2 + H2O
-
 Pseudomonas sp. (1-deoxy-D-fructos-1-yl)amine + hexanal + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 106000, SDS-PAGE Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
 5.1