Protein Variants | Comment | Organism |
---|---|---|
C314A | mutation of the covalent attachment site of FAD, mutant forms unstable complexes with FAD. In situ reconstitution of activity by assaying mutant in presence of FAD or 8-nor-8-chloro-FAD, giving a specific activity of 14% or 80% of wild-type, resp. Mutant exhibits high affinity for reduced flavin | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-deaza-FAD | competitive inhibition of flavinylation of apoenzyme | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.1 | - |
pH 8.0, 25°C, mutant C315A, presence of FAD | Bacillus sp. (in: Bacteria) |
23.2 | - |
pH 8.0, 25°C, mutant C315A, presence of 8-nor-8-chloro-FAD | Bacillus sp. (in: Bacteria) |
44.8 | - |
pH 8.0, 25°C, native enzyme | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | - |
Bacillus sp. (in: Bacteria) | glycine + formaldehyde + H2O2 | - |
? |