Cloned (Comment) | Organism |
---|---|
bifunctional LKR/SDH cDNA and monofunctional LKR cDNA are cloned, expression in Saccharomyces cerevisiae mutant lys1, but LKR cannot complement yeast LKR null mutant because of its uni-directional catabolic activity, overexpression in wild type yeast | Arabidopsis thaliana |
gene lys1 encoding lysine 2-oxoglutarate reductase is cloned | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant lys1 without lysine 2-oxoglutarate reductase activity | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | Saccharomyces cerevisiae | biosynthetic pathway of lysine in yeast and fungi | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
r | |
L-lysine + 2-oxoglutarate + NADPH | Arabidopsis thaliana | uni-directional activity plays an important role in regulating the catabolic function of alpha-aminoadipidic acid pathway in plants and stems from structure rather than its linkage to saccharopine dehydrogenase EC 1.5.1.9 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
ir | |
L-lysine + 2-oxoglutarate + NADPH | Arabidopsis thaliana | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
ir | |
L-lysine + 2-oxoglutarate + NADPH | Saccharomyces cerevisiae | lysine anabolic function is regulated by complex transcriptional and post-transcriptional controls | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Saccharomyces cerevisiae | - |
strains 8973b and 8989c, mutant lys1, lacking LKR activity, used as expression system for enzyme from Arabidopsis thaliana, lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 are two separate proteins | - |
Purification (Comment) | Organism |
---|---|
partial purification of a recombinant bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities and of the monofunctional lysine-2-oxoglutarate reductase domain | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine synthesis from saccharopine, reverse direction is favoured | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | NAD+ as electron acceptor for L-lysine formation | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine synthesis from saccharopine, reverse direction is favoured | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | NAD+ as electron acceptor for L-lysine formation | r | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine synthesis from saccharopine, reverse direction is favoured | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine synthesis from saccharopine, reverse direction is favoured | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Arabidopsis thaliana | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ir | |
L-lysine + 2-oxoglutarate + NADPH | unidirectional lysine catabolic activity | Arabidopsis thaliana | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ir | |
L-lysine + 2-oxoglutarate + NADPH | biosynthetic pathway of lysine in yeast and fungi | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
r | |
L-lysine + 2-oxoglutarate + NADPH | uni-directional activity plays an important role in regulating the catabolic function of alpha-aminoadipidic acid pathway in plants and stems from structure rather than its linkage to saccharopine dehydrogenase EC 1.5.1.9 | Arabidopsis thaliana | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
ir | |
L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Arabidopsis thaliana | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
ir | |
L-lysine + 2-oxoglutarate + NADPH | lysine anabolic function is regulated by complex transcriptional and post-transcriptional controls | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
r | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Arabidopsis thaliana | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | His-tagged LKRp, L-lysine catabolic activity | Arabidopsis thaliana |
9.5 | - |
L-lysine anabolic activity | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |