Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-ornithine | complete inhibition of saccharopine formation, competitive with lysine | Rattus norvegicus | |
leucine | complete inhibition of saccharopine formation, competitive with lysine | Rattus norvegicus | |
tryptophan | complete inhibition of saccharopine formation | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
NADPH | - |
Rattus norvegicus | |
1.4 | - |
2-oxoglutarate | - |
Rattus norvegicus | |
2.2 | - |
L-lysine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9, SDS-PAGE | Rattus norvegicus |
230000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar strain | - |
Rattus norvegicus male Wistar | - |
male Wistar strain | - |
Purification (Comment) | Organism |
---|---|
300fold purification, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.3 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, 10 mM Tris-HCl buffer, pH 7.6, 5 mM 2-mercaptoethanol, 0.1 mM EDTA, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | lysine formation is only 3-5% of the saccharopine formation | r | |
L-lysine + 2-oxoglutarate + NADPH | very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r | |
L-lysine + 2-oxoglutarate + NADPH | equilibrium favors sacharopine formation, lysine formation is only 3-5% of the saccharopine formation | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | lysine formation is only 3-5% of the saccharopine formation | r | |
L-lysine + 2-oxoglutarate + NADPH | equilibrium favors sacharopine formation, lysine formation is only 3-5% of the saccharopine formation | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | lysine formation is only 3-5% of the saccharopine formation | r | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r | |
L-lysine + 2-oxoglutarate + NADPH | very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH | Rattus norvegicus male Wistar | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | lysine formation is only 3-5% of the saccharopine formation | r | |
L-lysine + 2-oxoglutarate + NADPH | very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH | Rattus norvegicus male Wistar | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r | |
additional information | not as electron acceptor: NADH | Rattus norvegicus | ? | - |
? | |
additional information | lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 are separate enzymes | Rattus norvegicus | ? | - |
? | |
additional information | not as substrates: ornithine, glutamine, oxaloacetate, pyruvate | Rattus norvegicus | ? | - |
? | |
additional information | not as electron acceptor: NADH | Rattus norvegicus male Wistar | ? | - |
? | |
additional information | lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 are separate enzymes | Rattus norvegicus male Wistar | ? | - |
? | |
additional information | not as substrates: ornithine, glutamine, oxaloacetate, pyruvate | Rattus norvegicus male Wistar | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 52000, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
saccharopine formation | Rattus norvegicus |
9 | - |
L-lysine formation | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADPH | specific for NADPH | Rattus norvegicus |