Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | substrate inhibition | Escherichia coli | |
NADH | substrate inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
5,10-methylenetetrahydrofolate | pH 7.2, 15°C | Escherichia coli | |
0.013 | - |
NADH | pH 7.2, 15°C | Escherichia coli | |
0.075 | - |
5-methyltetrahydrofolate | pH 7.2, 15°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
119000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEZ1 | cf. EC 1.5.1.20 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, 50 mM phosphate buffer, pH 7.2, 10% glycerol, 0.3 mM EDTA, stable for up to one year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + menadiol | - |
Escherichia coli | 5-methyltetrahydrofolate + menadione | - |
r | |
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Escherichia coli | 5-methyltetrahydrofolate + NAD+ | - |
? | |
5-methyltetrahydrofolate + menadione | - |
Escherichia coli | 5,10-methylenetetrahydrofolate + menadiol | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33100, calculated from sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
metF | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
NADH | pH 7.2, 15°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 FAD per subunit | Escherichia coli | |
additional information | no cofactor: NADPH | Escherichia coli | |
NADH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
5,10-methylenetetrahydrofolate | pH 7.2, 15°C | Escherichia coli | |
0.009 | - |
NADH | pH 7.2, 15°C | Escherichia coli |