Literature summary for 1.5.1.53 extracted from

Bezerra, G.A.; Holenstein, A.; Foster, W.R.; Xie, B.; Hicks, K.G.; Buerer, C.; Lutz, S.; Mukherjee, A.; Sarkar, D.; Bhattacharya, D.; Rutter, J.; Talukdar, A.; Brown, P.J.; Luo, M.; Shi, L.; Froese, D.S.; Yue, W.W.
Identification of small molecule allosteric modulators of 5,10-methylenetetrahydrofolate reductase (MTHFR) by targeting its unique regulatory domain (2021), Biochimie, 183, 100-107 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	expression of constructs encompassing the full-length, just the catalytic and regulatory domain (amino acids 38-644), and encompassing the regulatory domain and part of the linker region (amino acids 348-656)	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
(S)-SKI-72	presence of (S)-SKI-72 leads to a Tm shift of approximately 10 degrees	Homo sapiens
S-adenosyl-L-methionine	presence of S-adenosyl-L-methionine leads to a Tm shift of about 4 degrees	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P42898	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293 cell	-	Homo sapiens	-

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
48	-	melting temperature	Homo sapiens
53	-	melting temperature, presence of S-adenosyl-L-methionine	Homo sapiens
58	-	melting temperature, presence of (S)-SKI-72	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00078	-	(S)-SKI-72	pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.0058	-	S-adenosyl-L-methionine	pH not specified in the publication, temperature not specified in the publication	Homo sapiens

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Release 2023.1 (January 2023)