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Literature summary for 1.5.1.42 extracted from

  • Gerlo, E.; Charlier, J.
    Identification of NADH-specific and NADPH-specific FMN reductases in Beneckea harveyi (1975), Eur. J. Biochem., 57, 461-467.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
FMN pH 6.8, 23°C Vibrio harveyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
gel filtration Vibrio harveyi
31000
-
sucrose density gradient centrifugation Vibrio harveyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FMN + NADH + H+ Vibrio harveyi
-
FMNH2 + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vibrio harveyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.05
-
pH 6.8, 23°C Vibrio harveyi

Storage Stability

Storage Stability Organism
-20°C, considerable loss of activity of reductase preparations Vibrio harveyi
4°C, considerable loss of activity of reductase preparations Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADH + H+
-
Vibrio harveyi FMNH2 + NAD+
-
?
FMN + NADH + H+ the affinity of the NADH-specific reductase is about 60 times greater for NADH than for NADPH Vibrio harveyi FMNH2 + NAD+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Vibrio harveyi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
5 min, 60% loss of activity Vibrio harveyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
NADH the affinity of the NADH-specific reductase is about 60 times greater for NADH than for NADPH Vibrio harveyi