KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
FMN | pH 6.8, 23°C | Vibrio harveyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
gel filtration | Vibrio harveyi |
31000 | - |
sucrose density gradient centrifugation | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADH + H+ | Vibrio harveyi | - |
FMNH2 + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio harveyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.05 | - |
pH 6.8, 23°C | Vibrio harveyi |
Storage Stability | Organism |
---|---|
-20°C, considerable loss of activity of reductase preparations | Vibrio harveyi |
4°C, considerable loss of activity of reductase preparations | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADH + H+ | - |
Vibrio harveyi | FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | the affinity of the NADH-specific reductase is about 60 times greater for NADH than for NADPH | Vibrio harveyi | FMNH2 + NAD+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Vibrio harveyi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min, 60% loss of activity | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the affinity of the NADH-specific reductase is about 60 times greater for NADH than for NADPH | Vibrio harveyi |