BRENDA - Enzyme Database
show all sequences of 1.5.1.41

NAD(P)H:flavin oxidoreductase of Escherichia coli

Fontecave, M.; Eliasson, R.; Reichard, P.; J. Biol. Chem. 262, 12325-12331 (1987)

Data extracted from this reference:

General Stability
General Stability
Organism
dilute solutions can be stabilized by the addition of bovine serum albumin (0.1 mg/ml) and/or by glycerol
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetate
-
Escherichia coli
additional information
no inhibition by EDTA, desferal or dicoumarol
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0006
-
riboflavin
second substrate: NADH, pH 7.5, 23C
Escherichia coli
0.0008
-
FAD
second substrate: NADH, pH 7.5, 23C
Escherichia coli
0.0008
-
FMN
second substrate: NADH, pH 7.5, 23C
Escherichia coli
0.0016
-
riboflavin
second substrate: NADPH, pH 7.5, 23C
Escherichia coli
0.002
-
FMN
second substrate: NADPH, pH 7.5, 23C
Escherichia coli
0.025
-
NADH
second substrate riboflavin, pH 7.5, 23C
Escherichia coli
0.043
-
NADPH
second substrate riboflavin, pH 7.5, 23C
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
gel filtration
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.36
-
pH 7.5, 23C
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
FAD + NADH + H+
no activity with FAD + NADPH
392190
Escherichia coli
FADH2 + NAD+
-
-
-
?
FMN + NADH + H+
-
392190
Escherichia coli
FMNH2 + NAD+
-
-
-
?
FMN + NADPH + H+
very low activity
392190
Escherichia coli
FMNH2 + NADP+
-
-
-
?
riboflavin + NADH + H+
-
392190
Escherichia coli
reduced riboflavin + NAD+
-
-
-
?
riboflavin + NADPH + H+
-
392190
Escherichia coli
reduced riboflavin + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 28000-29000, SDS-PAGE
Escherichia coli
Synonyms
Synonyms
Commentary
Organism
NAD(P)H:flavin oxidoreductase
-
Escherichia coli
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
23
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.4
-
stable at pH 7.4, except at low protein concentrations
Escherichia coli
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Escherichia coli
isoelectric focusing
-
4.9
General Stability (protein specific)
General Stability
Organism
dilute solutions can be stabilized by the addition of bovine serum albumin (0.1 mg/ml) and/or by glycerol
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetate
-
Escherichia coli
additional information
no inhibition by EDTA, desferal or dicoumarol
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0006
-
riboflavin
second substrate: NADH, pH 7.5, 23C
Escherichia coli
0.0008
-
FAD
second substrate: NADH, pH 7.5, 23C
Escherichia coli
0.0008
-
FMN
second substrate: NADH, pH 7.5, 23C
Escherichia coli
0.0016
-
riboflavin
second substrate: NADPH, pH 7.5, 23C
Escherichia coli
0.002
-
FMN
second substrate: NADPH, pH 7.5, 23C
Escherichia coli
0.025
-
NADH
second substrate riboflavin, pH 7.5, 23C
Escherichia coli
0.043
-
NADPH
second substrate riboflavin, pH 7.5, 23C
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.36
-
pH 7.5, 23C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
FAD + NADH + H+
no activity with FAD + NADPH
392190
Escherichia coli
FADH2 + NAD+
-
-
-
?
FMN + NADH + H+
-
392190
Escherichia coli
FMNH2 + NAD+
-
-
-
?
FMN + NADPH + H+
very low activity
392190
Escherichia coli
FMNH2 + NADP+
-
-
-
?
riboflavin + NADH + H+
-
392190
Escherichia coli
reduced riboflavin + NAD+
-
-
-
?
riboflavin + NADPH + H+
-
392190
Escherichia coli
reduced riboflavin + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 28000-29000, SDS-PAGE
Escherichia coli
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
23
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.4
-
stable at pH 7.4, except at low protein concentrations
Escherichia coli
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Escherichia coli
isoelectric focusing
-
4.9
Other publictions for EC 1.5.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743224
Man
Improvement of the intracellu ...
Corynebacterium crenatum, Corynebacterium crenatum SYPA5-5
Metab. Eng.
38
310-321
2016
-
-
1
-
1
-
-
5
-
-
-
2
-
5
-
-
1
-
-
-
-
-
6
-
3
1
-
-
5
1
-
-
4
-
-
-
-
-
1
4
-
1
-
-
-
-
5
-
-
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
5
1
-
-
-
-
-
-
-
5
5
720976
Okai
High pressure refolding, purif ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Protein Expr. Purif.
84
214-218
2012
-
-
1
1
-
-
-
-
-
-
-
-
-
13
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392202
Ingelman
Crystal structure of NAD(P)H:f ...
Escherichia coli
Biochemistry
38
7040-7049
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392192
Spyrou
Characterization of the flavin ...
Escherichia coli
J. Bacteriol.
173
3673-3679
1991
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
392190
Fontecave
NAD(P)H:flavin oxidoreductase ...
Escherichia coli
J. Biol. Chem.
262
12325-12331
1987
-
-
-
-
-
1
3
7
-
-
1
-
-
1
-
-
1
-
-
-
1
-
5
1
1
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
1
-
3
-
7
-
-
1
-
-
-
-
1
-
-
1
-
5
1
1
-
-
-
1
-
1
1
-
-
-
-
-
-