BRENDA - Enzyme Database
show all sequences of 1.5.1.25

Bovine brain ketimine reductase

Nardini, M.; Ricci, G.; Vesci, L.; Pecci, L.; Cavallini, D.; Biochim. Biophys. Acta 957, 286-292 (1988)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADH
reaction with DELTA1-piperidine 2-carboxylate
Bos taurus
0.24
-
S-Aminoethylcysteine ketimine
reaction with NADH
Bos taurus
0.26
-
NADH
reaction with DELTA1-piperidine 2-carboxylate
Bos taurus
0.33
-
DELTA1-Piperidine 2-carboxylate
reaction with DELTA1-piperidine-2-carboxylate
Bos taurus
1.17
-
Lanthionine ketimine
reaction with NADPH
Bos taurus
10
-
Cystathionine ketimine
reaction with NADPH
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Bos taurus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Bos taurus
100000
-
non-denaturing PAGE
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Reaction
Reaction
Commentary
Organism
thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H + H+
classical ping-pong mechanism
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
localized prevalently in cerebellum and cerebral cortices
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
16.6
-
-
Bos taurus
Storage Stability
Storage Stability
Organism
4°C, purified enzyme is stable for at least 1 month
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cystathionine ketimine + NADH
-
440359
Bos taurus
cyclothionine + NAD+
-
-
-
?
cystathionine ketimine + NADPH
-
440359
Bos taurus
cyclothionine + NADP+
-
-
-
?
DELTA1-piperideine 2-carboxylate + NADH
-
440359
Bos taurus
? + NAD+
-
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH
-
440359
Bos taurus
? + NADP+
-
-
-
?
lanthionine ketimine + NADH
-
440359
Bos taurus
1,4-thiomorpholine 3,5-dicarboxylic acid + NAD+
-
-
-
?
lanthionine ketimine + NADPH
-
440359
Bos taurus
1,4-thiomorpholine 3,5-dicarboxylic acid + NADP+
-
-
-
?
S-aminoethylcysteine ketimine + NADH
-
440359
Bos taurus
1,4-thiomorpholine 3-carboxylic acid + NAD+
-
-
-
?
S-aminoethylcysteine ketimine + NADPH
-
440359
Bos taurus
1,4-thiomorpholine 3-carboxylic acid + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 45000, SDS-PAGE
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
reaction with lanthionine ketimine and NADPH
Bos taurus
5
-
reaction with S-aminoethylcysteine ketimine and NADH, cystathionine ketimine and NADPH or 1-piperidine 2-carboxylate and NADPH
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
reduction rate for cystathionine ketimine, lanthionine ketimine and DELTA1-piperidine 2-carboxylate is higher with NADPH than with NADH. Reduction rate for S-aminoethylcysteine with NADH is higher than with NADPH
Bos taurus
NADPH
reduction rate for cystathionine ketimine, lanthionine ketimine and DELTA1-piperidine 2-carboxylate is higher with NADPH than with NADH. Reduction rate for S-aminoethylcysteine with NADH is higher than with NADPH
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
reduction rate for cystathionine ketimine, lanthionine ketimine and DELTA1-piperidine 2-carboxylate is higher with NADPH than with NADH. Reduction rate for S-aminoethylcysteine with NADH is higher than with NADPH
Bos taurus
NADPH
reduction rate for cystathionine ketimine, lanthionine ketimine and DELTA1-piperidine 2-carboxylate is higher with NADPH than with NADH. Reduction rate for S-aminoethylcysteine with NADH is higher than with NADPH
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADH
reaction with DELTA1-piperidine 2-carboxylate
Bos taurus
0.24
-
S-Aminoethylcysteine ketimine
reaction with NADH
Bos taurus
0.26
-
NADH
reaction with DELTA1-piperidine 2-carboxylate
Bos taurus
0.33
-
DELTA1-Piperidine 2-carboxylate
reaction with DELTA1-piperidine-2-carboxylate
Bos taurus
1.17
-
Lanthionine ketimine
reaction with NADPH
Bos taurus
10
-
Cystathionine ketimine
reaction with NADPH
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Bos taurus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Bos taurus
100000
-
non-denaturing PAGE
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
localized prevalently in cerebellum and cerebral cortices
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
16.6
-
-
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, purified enzyme is stable for at least 1 month
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cystathionine ketimine + NADH
-
440359
Bos taurus
cyclothionine + NAD+
-
-
-
?
cystathionine ketimine + NADPH
-
440359
Bos taurus
cyclothionine + NADP+
-
-
-
?
DELTA1-piperideine 2-carboxylate + NADH
-
440359
Bos taurus
? + NAD+
-
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH
-
440359
Bos taurus
? + NADP+
-
-
-
?
lanthionine ketimine + NADH
-
440359
Bos taurus
1,4-thiomorpholine 3,5-dicarboxylic acid + NAD+
-
-
-
?
lanthionine ketimine + NADPH
-
440359
Bos taurus
1,4-thiomorpholine 3,5-dicarboxylic acid + NADP+
-
-
-
?
S-aminoethylcysteine ketimine + NADH
-
440359
Bos taurus
1,4-thiomorpholine 3-carboxylic acid + NAD+
-
-
-
?
S-aminoethylcysteine ketimine + NADPH
-
440359
Bos taurus
1,4-thiomorpholine 3-carboxylic acid + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 45000, SDS-PAGE
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
reaction with lanthionine ketimine and NADPH
Bos taurus
5
-
reaction with S-aminoethylcysteine ketimine and NADH, cystathionine ketimine and NADPH or 1-piperidine 2-carboxylate and NADPH
Bos taurus
Other publictions for EC 1.5.1.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741584
Hallen
Ketimine reductase/CRYM catal ...
Homo sapiens
Amino Acids
47
2457-2461
2015
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
7
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
743354
Hallen
Insights into enzyme catalysi ...
Bos taurus, Homo sapiens, Mus musculus
Neurochem. Res.
40
1252-1266
2015
-
-
-
-
-
-
20
5
3
-
-
15
-
3
-
-
-
3
-
3
-
-
16
-
1
-
-
4
3
-
-
3
6
-
1
-
-
-
3
-
-
-
1
20
6
5
3
-
-
15
-
-
-
-
-
3
-
-
16
-
1
-
-
4
3
-
-
-
-
4
4
-
4
4
741580
Hallen
Lysine metabolism in mammalia ...
Bos taurus, Homo sapiens, Macropus giganteus, Mus musculus, Rattus norvegicus, Sus scrofa
Amino Acids
45
1249-1272
2013
-
-
-
-
-
-
6
-
6
-
-
9
-
6
-
-
4
-
-
16
1
-
9
-
-
-
-
-
1
-
-
6
1
-
-
-
-
-
6
-
-
-
-
6
1
-
6
-
-
9
-
-
-
4
-
16
1
-
9
-
-
-
-
-
1
-
-
-
2
14
14
2
-
-
440358
Nardini
Purification and characterizat ...
Sus scrofa
Eur. J. Biochem.
173
689-694
1988
-
-
-
-
-
1
1
4
-
-
2
-
-
3
-
-
1
1
-
1
1
-
8
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
2
-
-
1
-
1
-
4
-
-
2
-
-
-
-
1
-
1
1
-
8
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
440359
Nardini
Bovine brain ketimine reductas ...
Bos taurus
Biochim. Biophys. Acta
957
286-292
1988
-
-
-
-
-
-
-
6
1
-
2
-
-
1
-
-
1
1
-
1
1
1
8
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
6
1
-
2
-
-
-
-
1
-
1
1
1
8
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-