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Literature summary for 1.5.1.25 extracted from

  • Nardini, M.; Ricci, G.; Vesci, L.; Pecci, L.; Cavallini, D.
    Bovine brain ketimine reductase (1988), Biochim. Biophys. Acta, 957, 286-292.
    View publication on PubMed
Search for more literature for 1.5.1.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 NADH reaction with DELTA1-piperidine 2-carboxylate Bos taurus
0.24
-
 S-Aminoethylcysteine ketimine reaction with NADH Bos taurus
0.26
-
 NADH reaction with DELTA1-piperidine 2-carboxylate Bos taurus
0.33
-
 DELTA1-Piperidine 2-carboxylate reaction with DELTA1-piperidine-2-carboxylate Bos taurus
1.17
-
 Lanthionine ketimine reaction with NADPH Bos taurus
10
-
 Cystathionine ketimine reaction with NADPH Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Bos taurus
100000
-
 non-denaturing PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H + H+ classical ping-pong mechanism Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
brain localized prevalently in cerebellum and cerebral cortices Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.6
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
4°C, purified enzyme is stable for at least 1 month Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cystathionine ketimine + NADH
-
 Bos taurus cyclothionine + NAD+
-
 ?
cystathionine ketimine + NADPH
-
 Bos taurus cyclothionine + NADP+
-
 ?
DELTA1-piperideine 2-carboxylate + NADH
-
 Bos taurus ? + NAD+
-
 ?
DELTA1-piperideine 2-carboxylate + NADPH
-
 Bos taurus ? + NADP+
-
 ?
lanthionine ketimine + NADH
-
 Bos taurus 1,4-thiomorpholine 3,5-dicarboxylic acid + NAD+
-
 ?
lanthionine ketimine + NADPH
-
 Bos taurus 1,4-thiomorpholine 3,5-dicarboxylic acid + NADP+
-
 ?
S-aminoethylcysteine ketimine + NADH
-
 Bos taurus 1,4-thiomorpholine 3-carboxylic acid + NAD+
-
 ?
S-aminoethylcysteine ketimine + NADPH
-
 Bos taurus 1,4-thiomorpholine 3-carboxylic acid + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 reaction with lanthionine ketimine and NADPH Bos taurus
5
-
 reaction with S-aminoethylcysteine ketimine and NADH, cystathionine ketimine and NADPH or 1-piperidine 2-carboxylate and NADPH Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADH reduction rate for cystathionine ketimine, lanthionine ketimine and DELTA1-piperidine 2-carboxylate is higher with NADPH than with NADH. Reduction rate for S-aminoethylcysteine with NADH is higher than with NADPH Bos taurus
NADPH reduction rate for cystathionine ketimine, lanthionine ketimine and DELTA1-piperidine 2-carboxylate is higher with NADPH than with NADH. Reduction rate for S-aminoethylcysteine with NADH is higher than with NADPH Bos taurus