BRENDA - Enzyme Database show
show all sequences of 1.5.1.17

Anaerobiosis and metabolic plasticity of Pinna nobilis biochemical and ecological features

Lagana, G.; Barreca, D.; Giacobbe, S.; Bellocco, E.; Biochem. Syst. Ecol. 56, 138-143 (2014)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
the enzyme activity in adductor muscle decreases following the marine-brackish gradient
Pinna nobilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-alanine + pyruvate + NADH + H+
Pinna nobilis
-
beta-alanopine + NAD+ + H2O
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pinna nobilis
-
collected in the straits of Messina area (central Mediterranean) between May and June of 2011, from two nearby marine and brackish-water sites, two populations
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adductor muscle
the adductor muscle also shows a conspicuous amount of strombine dehydrogenase
Pinna nobilis
-
gill
-
Pinna nobilis
-
hepatopancreas
-
Pinna nobilis
-
mantle
-
Pinna nobilis
-
additional information
enzyme tissue distribution and expression analysis
Pinna nobilis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-alanine + pyruvate + NADH + H+
-
741868
Pinna nobilis
beta-alanopine + NAD+ + H2O
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pinna nobilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pinna nobilis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pinna nobilis
NADH
-
Pinna nobilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pinna nobilis
NADH
-
Pinna nobilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
the enzyme activity in adductor muscle decreases following the marine-brackish gradient
Pinna nobilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-alanine + pyruvate + NADH + H+
Pinna nobilis
-
beta-alanopine + NAD+ + H2O
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adductor muscle
the adductor muscle also shows a conspicuous amount of strombine dehydrogenase
Pinna nobilis
-
gill
-
Pinna nobilis
-
hepatopancreas
-
Pinna nobilis
-
mantle
-
Pinna nobilis
-
additional information
enzyme tissue distribution and expression analysis
Pinna nobilis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-alanine + pyruvate + NADH + H+
-
741868
Pinna nobilis
beta-alanopine + NAD+ + H2O
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pinna nobilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pinna nobilis
General Information
General Information
Commentary
Organism
metabolism
bivalves have evolved diverse and highly specialised strategies for surviving in hypoxic episodes including pathways that are efficient both in terms of ATP production, and in minimising H+ and toxic end product accumulation. Under these circumstances, glycogen is metabolized to pyruvate and the cytosolic NADH/NAD+ redox ratio is balanced by the reduction of pyruvate to lactate. Alternatively, NAD+ can be recycled more efficiently by coupling an amino acid to pyruvate, with formation of opines such as alanopine, tauropine, octopine, and strombine. Specimens utilizing the octopine rather than the alanopine pathway will increase energy flow rapidly, developing a major ability to counteract environmental variations. The high ratio between malate dehydrogenase/lactate dehydrogenase is due to the ability of Pinna nobilis to turn on anaerobic metabolism as a consequence of environmental or anthropogenic stresses. Anaerobic pathways are not all equivalent in terms of energy production based upon maximum rates for ATP output (lactate > octopine > alanopine = strombine)
Pinna nobilis
additional information
comparisons of opine dehydrogenases activities (octopine dehydrogenase, alanopine dehydrogenase, strombine dehydrogenase, and tauropine dehydrogenase) in the adductor muscle, overview. The ODH activity in adductor muscle increases following the marine-brackish gradient, while the one of ADH, SDH and TDH decreases following the same gradient
Pinna nobilis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
bivalves have evolved diverse and highly specialised strategies for surviving in hypoxic episodes including pathways that are efficient both in terms of ATP production, and in minimising H+ and toxic end product accumulation. Under these circumstances, glycogen is metabolized to pyruvate and the cytosolic NADH/NAD+ redox ratio is balanced by the reduction of pyruvate to lactate. Alternatively, NAD+ can be recycled more efficiently by coupling an amino acid to pyruvate, with formation of opines such as alanopine, tauropine, octopine, and strombine. Specimens utilizing the octopine rather than the alanopine pathway will increase energy flow rapidly, developing a major ability to counteract environmental variations. The high ratio between malate dehydrogenase/lactate dehydrogenase is due to the ability of Pinna nobilis to turn on anaerobic metabolism as a consequence of environmental or anthropogenic stresses. Anaerobic pathways are not all equivalent in terms of energy production based upon maximum rates for ATP output (lactate > octopine > alanopine = strombine)
Pinna nobilis
additional information
comparisons of opine dehydrogenases activities (octopine dehydrogenase, alanopine dehydrogenase, strombine dehydrogenase, and tauropine dehydrogenase) in the adductor muscle, overview. The ODH activity in adductor muscle increases following the marine-brackish gradient, while the one of ADH, SDH and TDH decreases following the same gradient
Pinna nobilis
Other publictions for EC 1.5.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
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1
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1
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1
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1
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1
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-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
1
-
1
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5
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1
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1
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1
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2
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2
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1
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1
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5
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
742962
Gohlke
Binding region of alanopine d ...
Arenicola marina
J. Chem. Inf. Model.
53
2493-2498
2013
-
-
-
-
-
-
1
3
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1
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1
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3
1
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3
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1
1
3
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1
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1
-
-
-
-
3
1
-
-
-
2
-
-
-
-
-
3
3
-
-
-
13290
Carvajal
-
Lactate dehydrogenase, alanopi ...
Concholepas concholepas
Comp. Biochem. Physiol. B
108
543-550
1994
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Azumapecten farreri nipponensis, Buccinum isaotakii, Cellana grata, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Haliotis discus hannai, Heterololigo bleekeri, Liolophura japonica, Littorina brevicula, Meretrix lusoria, Mesocentrotus nudus, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Asterias amurensis, no activity in Asterina pectinifera, no activity in Aurelia aurita, no activity in Balanus cariosus, no activity in Halichondria japonica, no activity in Halocynthia roretzi, no activity in Hemigrapsus sanguineus, no activity in Hexagrammos otakii, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pagurus samuelis, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, Octopus vulgaris, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Todarodes pacificus, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
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-
6
-
-
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-
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6
-
-
-
-
-
-
4
-
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-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
11
-
-
-
1
-
1
-
-
1
-
-
2
-
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10
-
-
-
-
-
-
-
-
2
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-
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-
2
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-
-
4
-
11
-
-
-
1
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-
-
1
-
2
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13366
Manchenko
Nonfluorescent negative stain ...
Polydora ciliata, Polydora commensalis, Polydora glycymerica, Pseudopolydora paucibranchiata
Anal. Biochem.
145
308-310
1985
-
4
-
-
-
-
-
-
-
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-
-
-
5
-
-
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-
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4
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8
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4
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8
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4
-
-
-
-
-
-
-
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-
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-
-
-
-
-
13368
Siegmund
Determination of meso-alanopin ...
Arenicola marina, Crassostrea angulata, Mytilus edulis, Nucula nitida
Hoppe-Seyler's Z. Physiol. Chem.
364
807-812
1983
-
-
-
-
-
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4
-
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1
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6
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4
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8
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8
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1
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6
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4
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-
-
13372
Plaxton
-
Purification and properties of ...
Busycotypus canaliculatum
Comp. Biochem. Physiol. B
76
321-326
1983
-
-
-
-
-
-
4
1
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-
2
-
1
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1
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3
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3
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6
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2
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2
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4
-
1
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2
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1
-
3
-
-
3
-
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-
-
-
6
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
7
17
2
-
1
-
-
1
-
-
1
-
-
3
1
1
12
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
17
2
-
1
-
-
-
-
1
-
3
1
1
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13371
Plaxton
-
Tissue specific isozymes of al ...
Busycotypus canaliculatum
Can. J. Zool.
60
1568-1572
1982
-
-
-
-
-
-
-
6
1
-
-
-
-
1
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7
-
-
2
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-
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-
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2
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2
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6
1
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7
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13374
Plaxton
-
Alanopine dehydrogenase: purif ...
Littorina littorea
J. Comp. Physiol.
149
57-65
1982
-
-
-
-
-
-
25
8
-
-
2
1
-
1
-
-
1
-
-
1
1
-
9
1
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-
4
-
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2
-
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-
2
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25
-
8
-
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2
1
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1
-
1
1
-
9
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
13375
Baldwin
-
The properties and functions o ...
Bursa sp., Cantharus undosus, Conomurex luhuanus, Conus arenatus, Cymatium pileare, Cypraea tigris, Cypraecassis rufa, Lambis lambis, Lambis millepeda, Lambis scorpius, Menathais tuberosa, Mitra eremitarum, Nassarius coronatus, Nassarius glans particeps, Nerita atramentosa, Strombus aurisdianae, Strombus canarium, Strombus gibberulus, Strombus labiatus, Strombus lentiginosus, Strombus sinuatus, Tibia martinii, Xenophora crispa
Pac. Sci.
36
381-394
1982
-
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4
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1
1
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23
-
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1
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4
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2
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46
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46
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4
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1
1
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1
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4
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2
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-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
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4
1
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1
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1
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1
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6
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2
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2
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2
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4
1
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1
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1
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6
-
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-
-
2
-
-
-
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-
-
-
-
13370
Fields
Purification and properties of ...
Crassostrea gigas
Eur. J. Biochem.
114
615-621
1981
-
-
-
-
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7
10
-
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2
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2
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1
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2
1
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8
1
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2
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2
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7
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10
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2
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1
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2
1
-
8
1
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-
-
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-
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-
13367
Fields
Alanopine and strombine are no ...
Crassostrea gigas
Arch. Biochem. Biophys.
201
110-114
1980
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1
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1
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